Phosphorus SAD Phasing for Nucleic Acid Structures: Limitations and Potential
| Autor: | Joel M. Harp, Pradeep S. Pallan, Martin Egli |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Diffraction General Chemical Engineering chemistry.chemical_element Crystal structure Biology 010403 inorganic & nuclear chemistry behavioral disciplines and activities 01 natural sciences Inorganic Chemistry Crystal 03 medical and health sciences Phase (matter) mental disorders lcsh:QD901-999 General Materials Science phosphorus Phosphorus SAD phasing nucleic acid Condensed Matter Physics Phaser 0104 chemical sciences Crystallography 030104 developmental biology Dodecameric protein chemistry behavior and behavior mechanisms Nucleic acid lcsh:Crystallography psychological phenomena and processes |
| Zdroj: | Crystals; Volume 6; Issue 10; Pages: 125 Crystals, Vol 6, Iss 10, p 125 (2016) |
| ISSN: | 2073-4352 |
| Popis: | Phasing of nucleic acid crystal diffraction data using the anomalous signal of phosphorus, P-SAD, at Cukα wavelength has been previously demonstrated using Z-DNA. Since the original work on P-SAD with Z-DNA there has been, with a notable exception, a conspicuous absence of applications of the technique to additional nucleic acid crystal structures. We have reproduced the P-SAD phasing of Z-DNA using a rotating-anode source and have attempted to phase a variety of nucleic acid crystals using P-SAD without success. A comparison of P-SAD using Z-DNA and a representative nucleic acid, the Dickerson-Drew dodecamer, is presented along with a S-SAD using only two sulfurs to phase a 2’-thio modified DNA decamer. A theoretical explanation for the limitation of P-SAD applied to nucleic acids is presented to show that the relatively high atomic displacement parameter of phosphorus in the nucleic acid backbone is responsible for the lack of success in applying P-SAD to nucleic acid diffraction data. |
| Databáze: | OpenAIRE |
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