GLP-2 Prevents Intestinal Mucosal Atrophy and Improves Tissue Antioxidant Capacity in a Mouse Model of Total Parenteral Nutrition
| Autor: | Xinying Wang, Xiao Wan, Jingcheng Bi, Qiucheng Lei, Chao Wu, Feng Tian, Huijun Zheng, Xuejin Gao, Tingting Jiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine antioxidant capacity Antioxidants Mice chemistry.chemical_compound Intestinal mucosa Glucagon-Like Peptide 2 Intestinal Mucosa Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins Nutrition and Dietetics biology Caspase 3 digestive oral and skin physiology apoptosis Glucagon-like peptide-2 Glutathione Up-Regulation Intestines Models Animal glucagon-like peptide-2 proliferation GRP78 Parenteral Nutrition Total lcsh:Nutrition. Foods and food supply medicine.medical_specialty lcsh:TX341-641 Article Superoxide dismutase 03 medical and health sciences Atrophy Proliferating Cell Nuclear Antigen Internal medicine medicine Animals Superoxide Dismutase medicine.disease Proliferating cell nuclear antigen 030104 developmental biology Endocrinology Parenteral nutrition chemistry Apoptosis Immunology biology.protein Food Science |
| Zdroj: | Nutrients; Volume 8; Issue 1; Pages: 33 Nutrients Nutrients, Vol 8, Iss 1, p 33 (2016) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu8010033 |
| Popis: | We investigated the effects of exogenous glucagon-like peptide-2 (GLP-2) on mucosal atrophy and intestinal antioxidant capacity in a mouse model of total parenteral nutrition (TPN). Male mice (6–8 weeks old) were divided into three groups (n = 8 for each group): a control group fed a standard laboratory chow diet, and experimental TPN (received standard TPN solution) and TPN + GLP-2 groups (received TPN supplemented with 60 µg/day of GLP-2 for 5 days). Mice in the TPN group had lower body weight and reduced intestinal length, villus height, and crypt depth compared to the control group (all p < 0.05). GLP-2 supplementation increased all parameters compared to TPN only (all p < 0.05). Intestinal total superoxide dismutase activity and reduced-glutathione level in the TPN + GLP-2 group were also higher relative to the TPN group (all p < 0.05). GLP-2 administration significantly upregulated proliferating cell nuclear antigen expression and increased glucose-regulated protein (GRP78) abundance. Compared with the control and TPN + GLP-2 groups, intestinal cleaved caspase-3 was increased in the TPN group (all p < 0.05). This study shows GLP-2 reduces TPN-associated intestinal atrophy and improves tissue antioxidant capacity. This effect may be dependent on enhanced epithelial cell proliferation, reduced apoptosis, and upregulated GRP78 expression. |
| Databáze: | OpenAIRE |
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