An involucrin-like protein in hepatocytes serves as a substrate for tissue transglutaminase during apoptosis
| Autor: | Vilmos A. Thomazy, Noémi Kedei, E Taresa, László Fésüs |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Programmed cell death
integumentary system biology Molecular mass Liver cytology Tissue transglutaminase Cell Biology Orvostudományok Biochemistry Molecular biology Cornified envelope medicine.anatomical_structure Apoptosis Hepatocyte medicine biology.protein Elméleti orvostudományok Molecular Biology Involucrin |
| Popis: | Cornified envelopes and apoptotic bodies are transglutaminase-cross-linked end-products of physiological cell death pathways. The two structures have similar amino acid composition. Involucrin has been considered as a cornified envelope precursor protein expressed specifically in terminally differentiating keratinocytes and squamous epithelia. We report the presence in hepatocytes of an involucrin-like protein which could be purified from dog liver with procedures characteristic to involucrins. When compared to purified dog esophagus involucrin, the liver protein also reacts with anti-involucrin antibodies, has the same relative molecular mass, possesses similar amino acid composition, and shows almost identical peptide mapping pattern. The involucrin-like protein is detectable by immunohistochemistry in normal and apoptotic hepatocytes, is a substrate of tissue transglutaminase, and is incorporated into cross-linked apoptotic bodies. These results suggest that there are overlapping molecular components in the two characteristic forms (cornification and apoptosis) of naturally occurring cell death. |
| Databáze: | OpenAIRE |
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