Selective cell culture of brain cells by serum-free, hormone-supplemented media: A comparative morphological study
| Autor: | Richard A. Wolfe, Don B. McClure, Gordon Sato, Gerd Brunner, Klaus Lang |
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| Rok vydání: | 1981 |
| Předmět: |
Cell type
Cell Biology Fibroblast growth factor Cell Line Neuroblastoma cell Mice Developmental Neuroscience Serum free Culture Techniques Glioma medicine Animals Nerve Growth Factors Mice Inbred BALB C Brain Cell Differentiation Rats Inbred Strains medicine.disease Hormones Culture Media Fibronectins Rats Cell biology Fibroblast Growth Factors medicine.anatomical_structure Cell culture Peptides Developmental Biology Hormone |
| Zdroj: | Developmental Brain Research. 2:563-575 |
| ISSN: | 0165-3806 |
| Popis: | We cultured single cell suspensions derived from enzymatically digested rat and mouse brains in the serum-free, hormone-supplemented media originally established for culturing glioma (C6) and neuroblastoma cell lines (B104). We succeeded in selecting different cell types by varying the hormones added to the culture medium. In addition to the medium the attachment factors used for coating the culture dishes proved important for the establishment of a selective culture system. In a comparative morphological study we show that different particular microenvironmental conditions determine the growth and/or survival of different cell types of dissociated brain cells. |
| Databáze: | OpenAIRE |
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