Molecular epidemiology of leprosy based on VNTR typing in Thailand
| Autor: | Janisara Rudeeaneksin, Patrick J. Brennan, Supanee Pasadorn, Atchariya Lukebua, Sukanya Wattanapokayakit, Krisada Mahotarn, Varalakshmi Vissa, Ajincholapan, Sopa Srisungnam, Miyako Kimura, Rama Murthy Sakamuri, Benjawan Phetsuksiri |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
DNA Bacterial Male Adolescent Minisatellite Repeats Biology Young Adult Tandem repeat Genotype Humans Child Genotyping General Environmental Science Aged Genetics Aged 80 and over Molecular Epidemiology Molecular epidemiology Genetic Variation Middle Aged Thailand Mycobacterium leprae Variable number tandem repeat Minisatellite Genetic marker Leprosy Multibacillary General Earth and Planetary Sciences Microsatellite Female Leprosy Paucibacillary |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 0305-7518 |
| Popis: | Recently about 500 new cases of leprosy have been reported each year in Thailand. In addition to a steady rate of new case detection, Thailand is in Southeast Asia where leprosy is endemic in neighbouring countries; therefore, strain differentiation could be useful in tracing origins and routes of infection, and general leprosy surveillance. To identify suitable markers for differentiation of M. leprae strains in different global geographic regions and to determine the applicability of a systematic genotyping method for tracing leprosy transmission, variable nucleotide tandem repeats (VNTRs) of 14 loci were evaluated using DNA extracts from a total of 97 skin biopsies and slit skin smear samples. The alleles per locus ranged from 2-26 providing adequate strain differentiation. Microsatellite loci (GAA)21, (AT)17 are highly polymorphic followed by (GTA)9, (AC)8a, (AC)8b, and (AC)9. The minisatellites 6-7, 21-3 and 27-5 exhibited a limited number of alleles. The repeat of 23-3 showed no polymorphism. Overall, the strain types can be divided into two distinct Thai groups, according to the alleles at the (GGT)5 and 21-3 loci. However, there are no obvious geographical patterns of distribution of VNTR strain types. Closely matched VNTR profiles found in household members of two multi-case families suggested infection through a common source. |
| Databáze: | OpenAIRE |
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