Artificially generated dendritic cells misdirect antiviral immune responses
| Autor: | Patricia A. Johnson, Robert W. Newman, Cariosa M. Noone, Ellen Manahan |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
CD3 Complex
viruses medicine.medical_treatment T cell CD3 Immunology chemical and pharmacologic phenomena Apoptosis Respirovirus Infections Monocytes Cell Line Tissue Culture Techniques Immune system In vivo medicine Immunology and Allergy Humans Cell Proliferation Immunity Cellular biology Interferon-alpha Cell Differentiation Cell Biology Dendritic Cells Phenotype Virology Antigens Differentiation Cytokine medicine.anatomical_structure biology.protein Cytokines Cytokine secretion Interleukin-4 |
| Zdroj: | Journal of leukocyte biology. 81(4) |
| ISSN: | 0741-5400 |
| Popis: | Dendritic cells (DCs) are critical to the outcome of many viral infections. Questions still remain as to the relevance of artificially generated DCs in models of in vivo immune responses. We compared different DC generation pathways, in terms of phenotypic expression, cytokine production, apoptosis, and T cell proliferation, following viral infection. Direct viral infection of monocytes or monocytes cultured with supernatants from virally infected lung epithelial cells (A549 DCs) induce distinct DC subsets compared with viral infection of artificially generated IL-4 DCs and IFN-DCs. These virally infected DC subsets stimulated different cytokine secretion profiles and displayed contrasting sensitivities to viral-induced apoptosis. It is most interesting that we observed marked differences in the proliferation of purified CD3+ T cells from the virally infected DC subsets. In conclusion, artificially generated DCs skew immune responses to viral infections, and direct viral infection of monocytes and DCs, generated from monocytes cultured with supernatants from infected epithelial cells, appears to be a more relevant pathway of producing DCs, which mimic those generated in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text