Long non-coding RNA-Low Expression in Tumor inhibits the invasion and metastasis of esophageal squamous cell carcinoma by regulating p53 expression

Autor: Teng Ma, Yang Xia, Bin Liu, Chun‑Feng Pan, Peng‑Li Wang, Yi‑Jiang Chen
Rok vydání: 2016
Předmět:
p53
Male
0301 basic medicine
Cancer Research
Esophageal Neoplasms
esophageal squamous cell carcinoma cells
Cell
Apoptosis
migration
medicine.disease_cause
Biochemistry
Metastasis
0302 clinical medicine
lentivirus
Cell Movement
Neoplasm Metastasis
Articles
Middle Aged
Cell cycle
invasion
Long non-coding RNA
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Molecular Medicine
Female
RNA
Long Noncoding
Esophageal Squamous Cell Carcinoma
Adult
long non-coding RNA-Low Expression in Tumor
Biology
03 medical and health sciences
Cell Line
Tumor
Genetics
medicine
Humans
Neoplasm Invasiveness
Molecular Biology
Aged
Cell Proliferation
Neoplasm Staging
Oncogene
Cell growth
medicine.disease
Molecular biology
030104 developmental biology
Tumor progression
Neoplasm Grading
Tumor Suppressor Protein p53
Carcinogenesis
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2016.4913
Popis: Long non-coding RNAs (lncRNAs) are involved in governing fundamental biological processes, and, in many lncRNAs, the expression level is altered and likely to have a functional role in tumorigenesis, including apoptosis, migration and invasion. The lncRNA‑Low Expression in Tumor (LET), a recently identified lncRNA, was demonstrated to be downregulated in hepatocellular and gallbladder cancer. However, its role in esophageal squamous cell carcinoma (ESCC) requires investigation. The expression level of lncRNA‑LET mRNA in primary ESCC and matched healthy tissues (48 cases) was determined by reverse transcription‑quantitative polymerase chain reaction. In addition, the effects of lncRNA-LET on cell apoptosis were evaluated by flow cytometric analysis, the regulatory effect of lncRNA‑LET on migration was detected using a wound healing assay and cellular invasion was analyzed by Matrigel‑coated transwell assay. Furthermore, the effect of lncRNA‑LET on cell proliferation was investigated by 5‑ethynyl‑2'-deoxyuridine cell proliferation assay and protein levels of lncRNA-LET targets were analyzed by western blotting. lncRNA-LET expression was decreased in primary ESCC tissues when compared with paired healthy tissues, and was identified to be associated with the clinical features. Overexpression of lncRNA‑LET was observed to inhibit the migration and invasion of ESCC cells, and modulate p53 expression levels in human ESCC cell lines in vitro. These results establish that lncRNA-LET is significant in the regulation of tumor progression and metastasis, and serves as a tumor suppressor in, and therefore has therapeutic potential for, the treatment of human ESCC.
Databáze: OpenAIRE
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