Associations of interleukin (IL)-1β, IL-1 receptor antagonist, and IL-10 with dental caries
| Autor: | Ferda Ozkinay, Cemal Eronat, Yasemin Ozdemir, Gulcin Itirli Aslan, Necil Kutukculer, Dilsah Cogulu, Hüseyin Onay |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2015 |
| Předmět: |
Male
Saliva medicine.drug_class Interleukin-1beta Dentistry Enzyme-Linked Immunosorbent Assay Dental Caries Polymerase Chain Reaction Pathogenesis Streptococcus mutans IL-1ra stomatognathic system IL-1 beta Medicine Humans IL-1ß Statistical analysis Child General Dentistry biology business.industry DMF Index ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS Interleukin biology.organism_classification Receptor antagonist Interleukin-10 ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS stomatognathic diseases Interleukin 10 Interleukin 1 Receptor Antagonist Protein ComputingMethodologies_PATTERNRECOGNITION Immunology IL-10 Female Analysis of variance InformationSystems_MISCELLANEOUS business |
| Zdroj: | Journal of oral science. 57(1) |
| ISSN: | 1880-4926 0003-5165 |
| Popis: | WOS: 000351657900005 PubMed ID: 25807906 Streptococcus mutans is important in dental caries. Although the role of cytokines in the pathogenesis of dental caries is not clear, components of Streptococcus mutans were found to stimulate production of pro-inflammatory cytokines. We examined the associations of interleukin (IL)-1 beta, IL-1 receptor antagonist (IL-1ra), and IL-10 with dental caries. Unstimulated whole saliva and blood samples were obtained from 108 children aged 6-12 years with high caries (decayed, missing, or filled teeth [dmft/DMFT] index >4, n = 37), moderate caries (dmft/DMFT = 1-4, n = 37), or caries-free (dmft/DMFT = 0, n = 34). Streptococcus mutans level was classified as low (= 10(5) CFU/mL). Saliva and serum concentrations of IL-1 beta, IL-1ra, and IL-10 were determined by ELISA. IL-1 beta, IL-1ra and IL-10 gene polymorphisms were genotyped using PCR and restriction fragment length polymorphism analysis. The chi-square, Mann-Whitney U, one-way ANOVA, posthoc, Fisher's exact, and t tests were used in statistical analysis. Dental caries was not correlated with salivary or serum concentrations of the studied cytokines. Streptococcus mutans level positively correlated with saliva IL-1 beta concentration and inversely correlated with saliva IL-1ra concentration. There was no correlation of IL-1 beta, IL-1ra, or IL-10 gene polymorphisms with dental caries. Streptococcus mutans is important in stimulating saliva IL-1 beta and inhibiting IL-1ra. Future studies of associations between cytokines and dental caries should investigate additional cytokines and enroll a larger number of participants. |
| Databáze: | OpenAIRE |
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