Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease

Autor:	Scott J. Gratz, Danielle C. Hamm, Jill Wildonger, Alexander M. Cummings, Melissa M. Harrison, Jennifer N. Nguyen, Kate M. O'Connor-Giles, Laura K. Donohue
Rok vydání:	2013
Předmět:	Genome Insect homologous recombination Biology Genome engineering 03 medical and health sciences 0302 clinical medicine Notes Genetics CRISPR Animals Drosophila Proteins CRISPR RNA Clustered Regularly Interspaced Short Palindromic Repeats Cas9 Gene Germ-Line Mutation 030304 developmental biology 0303 health sciences Nuclease CRISPR interference Endodeoxyribonucleases fungi Methods Technology and Resources RNA Gene Targeting biology.protein Drosophila CRISPR-Cas Systems Genetic Engineering genome engineering 030217 neurology & neurosurgery Drosophila Protein
Zdroj:	Genetics
ISSN:	1943-2631
Popis:	We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bf804517bfddd454c892887f2e47b86 https://pubmed.ncbi.nlm.nih.gov/23709638 Zobrazit plný text záznamu