RBM4 Modulates Radial Migration via Alternative Splicing of Dab1 during Cortex Development
| Autor: | Woan-Yuh Tarn, Kuan-Yang Hung, Dhananjaya D |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Neurogenesis Subventricular zone Nerve Tissue Proteins Cell Line 03 medical and health sciences Exon Mice Cell Movement medicine Animals Humans Protein Isoforms Reelin Molecular Biology Cerebral Cortex Mice Knockout Neurons Gene knockdown biology Alternative splicing High-Throughput Nucleotide Sequencing RNA-Binding Proteins Cell Biology PTBP1 DAB1 Cell biology Alternative Splicing Reelin Protein 030104 developmental biology medicine.anatomical_structure Electroporation HEK293 Cells Gene Expression Regulation Cerebral cortex biology.protein Female Research Article |
| Popis: | The RNA-binding motif 4 (RBM4) protein participates in cell differentiation via its role in regulating the expression of tissue-specific or developmentally regulated mRNA splice isoforms. RBM4 is expressed in embryonic brain during development; it is initially enriched in the ventricular zone/subventricular zone and subsequently distributed throughout the cerebral cortex. Rbm4a knockout brain exhibited delayed migration of late-born neurons. Using in utero electroporation, we confirmed that knockdown of RBM4 impaired cortical neuronal migration. RNA immunoprecipitation with high-throughput sequencing identified Disabled-1 (Dab1), which encodes a critical reelin signaling adaptor, as a potential target of RBM4. Rbm4a knockout embryonic brain showed altered Dab1 isoform ratios. Overexpression of RBM4 promoted the inclusion of Dab1 exons 7 and 8 (7/8), whereas its antagonist polypyrimidine tract-binding protein 1 (PTBP1) acted in an opposite manner. RBM4 directly counteracted the effect of PTBP1 on exon 7/8 selection. Finally, we showed that the full-length Dab1, but not exon 7/8-truncated Dab1, rescued neuronal migration defects in RBM4-depleted neurons, indicating that RBM4 plays a role in neuronal migration via modulating the expression of Dab1 splice isoforms. Our findings imply that RBM4 is necessary during brain development and that its deficiency may lead to developmental brain abnormality. |
| Databáze: | OpenAIRE |
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