The In Vitro-In Vivo Safety Confirmation of PEG-40 Hydrogenated Castor Oil as a Surfactant for Oral Nanoemulsion Formulation
| Autor: | Heni Rachmawati, Olivia Mayasari Tandrasasmita, Kusnandar Anggadiredja, Raymond R. Tjandrawinata, Guntur Berlian, Sri Ayu, Miranti Anggraeni Novel |
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| Rok vydání: | 2016 |
| Předmět: |
PEG-40 hydrogenated castor oil
self-nanoemulsion glyceryl monooleate oral delivery cytotoxicity Pharmaceutical Science lcsh:RS1-441 02 engineering and technology Polyethylene glycol 010402 general chemistry 01 natural sciences Article lcsh:Pharmacy and materia medica Castor wax chemistry.chemical_compound Pulmonary surfactant In vivo PEG ratio Organic chemistry In vitro in vivo Chromatography Chemistry Aqueous two-phase system 021001 nanoscience & nanotechnology 0104 chemical sciences Particle size 0210 nano-technology |
| Zdroj: | Scientia Pharmaceutica Scientia Pharmaceutica; Volume 85; Issue 2; Pages: 18 Scientia Pharmaceutica, Vol 85, Iss 2, p 18 (2017) |
| ISSN: | 2218-0532 |
| Popis: | Evaluation on the safety use of high concentration of polyoxyl 40 (PEG-40) hydrogenated castor oil as a surfactant for oral nanoemulsion was performed in Webster mice. As previously reported, nearly 20% of PEG-40 hydrogenated castor oil was used to emulsify the glyceryl monooleate (GMO) as an oil to the aqueous phase. Thermodynamically stable and spontaneous nanoemulsion was formed by the presence of co-surfactant polyethylene glycol 400 (PEG-400). Standard parameters were analyzed for nanoemulsion including particle size and particle size distribution, the surface charge of nanoemulsion, and morphology. To ensure the safety of this nanoemulsion, several cell lines were used for cytotoxicity study. In addition, 5000 mg/kg body weight (BW) of the blank nanoemulsion was given orally to Webster mice once a day for 14 days. Several parameters such as gross anatomy, body weight, and main organs histopathology were observed. In particular, by considering the in vivo data, it is suggested that nanoemulsion composed with a high amount of PEG-40 hydrogenated castor oil is acceptable for oral delivery of active compounds. |
| Databáze: | OpenAIRE |
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