Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer
| Autor: | Julia Böttner, Milica Matijević, Irina Estrela-Lopis, Lela Korićanac, Luca Vannucci, Marijana Petković, Xinyue Liang, Zoran Šaponjić, Maja Nešić, Lenka Rajsiglova, Miloš Mojović, Milutin Stepić, Mila Vranješ, Đura Nakarada, Lan Mi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biocompatibility Cancer therapy medicine.medical_treatment Bioengineering Photodynamic therapy 02 engineering and technology 010402 general chemistry 01 natural sciences Immune system medicine General Materials Science Oxidative activity Cytotoxicity General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics In vitro 0104 chemical sciences 3. Good health TiO(2)prolate nanospheroids Nanomedicine Cell culture Modeling and Simulation Cancer cell Biophysics 0210 nano-technology Intracellular |
| Zdroj: | Journal of Nanoparticle Research |
| Popis: | TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. |
| Databáze: | OpenAIRE |
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