Proteomic Analyses of Pancreatic Cyst Fluids
| Autor: | Eileen Ke, James C. Watson, Xin Ming Li, Nancy A. Young, Minhhuyen Nguyen, Oleh Haluszka, Steven J. Cohen, David S. Weinberg, John P. Hoffman, Tiffany Liu, Bhavinkumar B. Patel, Samuel Litwin, Anthony T. Yeung, Hormoz Ehya, Jeffrey L. Tokar, Neal J. Meropol |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Proteomics Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Article Endocrinology Carcinoembryonic antigen parasitic diseases Internal Medicine medicine Humans Electrophoresis Gel Two-Dimensional Prospective Studies Aged Aged 80 and over Hepatology biology Chemistry Extramural Cyst Fluid Mucins Chromatography liquid Middle Aged medicine.disease Molecular biology Pancreatic cyst fluid Carcinoembryonic Antigen Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Pancreatic cyst Amylases biology.protein Electrophoresis Polyacrylamide Gel Female Pancreatic cysts Pancreatic Cyst Chromatography Liquid |
| Popis: | There are currently no diagnostic indicators that are consistently reliable, obtainable, and conclusive for diagnosing and risk-stratifying pancreatic cysts. Proteomic analyses were performed to explore pancreatic cyst fluids to yield effective diagnostic biomarkers.We have prospectively recruited 20 research participants and prepared their pancreatic cyst fluids specifically for proteomic analyses. Proteomic approaches applied were as follows: (1) matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry peptidomics with LC/MS/MS (HPLC-tandem mass spectrometry) protein identification; (2) 2-dimensional gel electrophoresis; (3) GeLC/MS/MS (tryptic digestion of proteins fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by LC/MS/MS).Sequencing of more than 350 free peptides showed that exopeptidase activities rendered peptidomics of cyst fluids unreliable; protein nicking by proteases in the cyst fluids produced hundreds of protein spots from the major proteins, making 2-dimensional gel proteomics unmanageable; GeLC/MS/MS revealed a panel of potential biomarker proteins that correlated with carcinoembryonic antigen (CEA).Two homologs of amylase, solubilized molecules of 4 mucins, 4 solubilized CEA-related cell adhesion molecules (CEACAMs), and 4 S100 homologs may be candidate biomarkers to facilitate future pancreatic cyst diagnosis and risk-stratification. This approach required less than 40 microL of cyst fluid per sample, offering the possibility to analyze cysts smaller than 1 cm in diameter. |
| Databáze: | OpenAIRE |
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