Effect of Alpha-Tocopherol and Dose Sensitivity on pancreatitis formation in Rats with Experimental Pancreatitis
| Autor: | Cüneyt Akyüz, Ayhan Özsoy, Deniz Tazeoğlu, Mehmet Gokceimam, Gulcin Kamali, Servet Rüştü Karahan |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty alpha-Tocopherol Gastroenterology Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Edema medicine Acinar cell Animals Amylase Rats Wistar Lipase Pancreas biology Tumor Necrosis Factor-alpha business.industry medicine.disease Rats Anesthesiology and Pain Medicine Pancreatitis chemistry Acute Disease Emergency Medicine biology.protein Acute pancreatitis Surgery Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Turkish Journal of Trauma and Emergency Surgery. |
| ISSN: | 1306-696X |
| Popis: | BACKGROUND Acute pancreatitis is an inflammatory disease accompanied by pancreatic inflammation characterized by acinar cell damage and leukocyte infiltration in the tissue. At present, mortality and morbidity rates are high despite the current treatment of pancreatitis; therefore, new studies and treatment studies are needed. In this study, the effects of alpha-tocopherol on different doses of L-arginine-induced experimental acute pancreatitis model were investigated. METHODS Thirty adult male Sprague-Dawley albino rats were randomly divided into four groups; control (sham) group (n=6), acute pancreatitis group (n=8), low-dose alpha-tocopherol (200 mg/kg once intraperitoneal [IP]) group (n=8), and high dose alpha-tocopherol (400 mg/kg once ip) group (n=8). Experimental acute pancreatitis model was created by a single IP dose of 5 g/kg of L-arginine. Alpha-tocopherol was administered in a single dose intraperitoneally, 30 min before the creation of the experimental model of acute pancreatitis induced by L-arginine induction in Groups 3 and 4. Tissue and blood samples were taken under anesthesia 72 h after L-arginine injection; then the rats were sacrificed by decapitation. Serum amylase, lipase, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-alpha, and C-reactive protein (CRP) levels were examined. Pancreatic tissue samples were examined under a light microscope for histopathological examination. RESULTS When the acute pancreatitis group (Group 2) was compared to the control group (Group 1), serum amylase, lipase, IL-1β, IL-6, TNF-alpha, and CRP levels were all significantly increased (p |
| Databáze: | OpenAIRE |
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