Optimization of elemental selenium (Se(0)) determination in yeasts by anion-exchange HPLC-ICP-MS
| Autor: | Hervé Martinez, Dominique Foix, Fabienne Séby, Véronique Vacchina, Mathieu Menta |
|---|---|
| Přispěvatelé: | Ultra Traces Analyses Aquitaine (UT2A), Ultra Traces Analyses Aquitaine, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
Anions
Chemical transformation media_common.quotation_subject chemistry.chemical_element 02 engineering and technology Chemical element 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry Selenium chemistry.chemical_compound X-ray photoelectron spectroscopy [CHIM.ANAL]Chemical Sciences/Analytical chemistry Yeasts XPS HPLC-ICP-MS [CHIM]Chemical Sciences Selenium Compounds Inductively coupled plasma mass spectrometry Chromatography High Pressure Liquid Sodium sulfite media_common Chromatography Photoelectron Spectroscopy 010401 analytical chemistry [CHIM.MATE]Chemical Sciences/Material chemistry Chromatography Ion Exchange 021001 nanoscience & nanotechnology Yeast Elemental selenium (Se(0)) 0104 chemical sciences Speciation speciation chemistry Calibration [SDE]Environmental Sciences Nanoparticles spICP-MS Powders 0210 nano-technology |
| Zdroj: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, Springer Verlag, 2021, 413, pp.1809-1816. ⟨10.1007/s00216-020-03129-y⟩ |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | International audience; An analytical method was developed for the speciation of elemental selenium (Se(0)) in selenized yeasts by anion-exchange HPLC-ICP-MS after its chemical transformation into SeSO32− by reaction with sodium sulfite. The presence of Se(0) in the yeasts was further confirmed by single-particle ICP-MS. Indeed, Se nanoparticles, if present, are expected to be, at least partly, Se(0). X-ray photoelectron spectroscopy, a well-recognized technique for chemical element speciation in the solid state, was also used with this objective. Both methods were able to confirm the presence of Se(0) in the selenized yeasts but failed to provide reliable quantitative results. Analytical performances of the HPLC-ICP-MS method were then evaluated for Se(0) determination. Quantification limits of 1 mg/kg were reached. The recovery levels from an added quantity comprised between 93 and 101%. Within-run and between-run precisions were both below 8%. The procedure developed was finally applied to quantify Se(0) content in a series of seven yeast batches from different suppliers. Se(0) was found to be present in all the studied yeasts and represented on average 10–15% of the total Se |
| Databáze: | OpenAIRE |
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