Detection of cocaine and its metabolites in amniotic fluid and umbilical cord tissue
| Autor: | Ruth E. Winecker, K Wobie, Fonda Davis Eyler, Marylou Behnke, Michael Conlon, Bruce A. Goldberger, Janet L. Karlix, Roger L. Bertholf, Ian R. Tebbett |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Narcotics Amniotic fluid Adolescent Health Toxicology and Mutagenesis Metabolite Physiology Toxicology Umbilical cord Gas Chromatography-Mass Spectrometry Analytical Chemistry Umbilical Cord chemistry.chemical_compound Cocaine Pregnancy medicine Environmental Chemistry Humans Maternal-Fetal Exchange Fetus Chemical Health and Safety Chromatography Prenatal cocaine exposure medicine.disease Amniotic Fluid Norcocaine medicine.anatomical_structure chemistry Benzoylecgonine Female |
| Zdroj: | Journal of analytical toxicology. 21(2) |
| ISSN: | 0146-4760 |
| Popis: | The increased use of cocaine by women of child-bearing age has left many health care scientists searching for improved methods of detecting prenatal cocaine exposure. To that end, a study of the determination of cocaine and its metabolites in amniotic fluid and umbilical cord tissue was undertaken. Amniotic fluid (n = 32) and umbilical cord tissue (n = 70) specimens were collected from pregnant subjects admitted to labor and delivery at Shands Hospital at the University of Florida (Gainesville, FL). Subjects were interviewed regarding drug use during each trimester. Subjects reporting cocaine use were designated as target subjects, and those denying use were control subjects. The specimens were subjected to solid-phase extraction and analyzed for cocaine and its metabolites by gas chromatography-mass spectrometry. Cocaine analytes (predominantly benzoylecgonine) were detected in 28.1 and 18.5% of the amniotic fluid and umbilical cord tissue specimens, respectively. Other cocaine analytes frequently detected included ecgonine methyl ester and m-hydroxy-benzoylecgonine in amniotic fluid specimens and ecgonine methyl ester, norcocaine, and m-hydroxybenzoylecgonine in umbilical cord tissue specimens. This study has shown that cocaine and its metabolites are readily detected in specimens of maternal and fetal origin. |
| Databáze: | OpenAIRE |
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