Inorganic polyphosphate in methylotrophic yeasts
| Autor: | N. A. Andreeva, Anton Zvonarev, M. A. Eldarov, L. P. Ryazanova, Tatiana Kulakovskaya, L. V. Trilisenko |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endopolyphosphatase activity Applied Microbiology and Biotechnology Pichia Pichia pastoris Transcriptome 03 medical and health sciences Polyphosphates Gene Expression Regulation Fungal otorhinolaryngologic diseases neoplasms Gene 030102 biochemistry & molecular biology biology Chemistry Gene Expression Profiling Methanol pathological conditions signs and symptoms General Medicine Metabolism biology.organism_classification digestive system diseases Yeast surgical procedures operative 030104 developmental biology Biochemistry Saccharomycetales Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 102:5235-5244 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need for phosphate, the decrease of long-chain polyP level, the accumulation of short-chain polyP, and enhanced endopolyphosphatase activity in the crude membrane fraction were observed in methanol-grown cells compared with glucose-grown cells of both species. Transcriptome analysis revealed notable differences in the expression patterns of key genes encoding proteins related to polyP metabolism. In methanol-grown cells, the genes encoding endopolyphosphatases and phosphate transporters were upregulated. The changes in polyP metabolism are probably related to the peculiarities of bioenergetics of methanol-grown cells. |
| Databáze: | OpenAIRE |
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