Hypothermia does not influence liver damage and function in a porcine polytrauma model

Autor: Eberhard Uhl, Florian Debus, Hans-Christoph Pape, Hinnerk Wulf, Julia Andruszkow, Steffen Ruchholtz, Nina Vogt, D. Eschbach, Klemens Horst, Frank Hildebrand, Thorsten Steinfeldt, Karsten Schöller, M. Sassen, Juliane Mohr, M. Frink
Přispěvatelé: University of Zurich, Eschbach, D
Rok vydání: 2017
Předmět:
Male
ypothermia
Swine
Blood volume
Wounds
Penetrating
Abdominal Injuries
1710 Information Systems
Wounds
Nonpenetrating
Random Allocation
0302 clinical medicine
Liver Function Tests
Hypothermia
Induced
Medicine
liver laceration
2718 Health Informatics
Trauma Severity Indices
medicine.diagnostic_test
Major trauma
Liver Diseases
Intensive Care Units
030220 oncology & carcinogenesis
Anesthesia
Musculoskeletal injury
medicine.symptom
Information Systems
Thoracic Injuries
Biomedical Engineering
Biophysics
2204 Biomedical Engineering
610 Medicine & health
Health Informatics
Bioengineering
Shock
Hemorrhagic
Biomaterials
03 medical and health sciences
Animals
1502 Bioengineering
business.industry
Multiple Trauma
2502 Biomaterials
030208 emergency & critical care medicine
Hypothermia
Liver Laceration
medicine.disease
Polytrauma
10021 Department of Trauma Surgery
Disease Models
Animal
trauma model
porcine animal model
Liver function
business
Liver function tests
1304 Biophysics
Zdroj: Technology and health care : official journal of the European Society for Engineering and Medicine. 26(2)
ISSN: 1878-7401
Popis: BACKGROUND: Previous studies revealed evidence that induced hypothermia attenuates ischemic organ injuries after severe trauma. In the present study, the effect of hypothermia on liver damage was investigated in a porcine long term model of multi-system injury, consisting of blunt chest trauma, penetrating abdominal trauma, musculoskeletal injury, and hemorrhagic shock METHODS: In 30 pigs, a standardized polytrauma including blunt chest trauma, penetrating abdominal trauma, musculoskeletal injury, and hemorrhagic shock of 45% of total blood volume was induced. Following trauma, hypothermia of 33∘C was induced for 12 h and intensive care treatment was evaluated for 48 h. As outcome parameters, we assessed liver function and serum transaminase levels as well as a histopathological analysis of tissue samples. A further 10 animals served as controls. RESULTS: Serum transaminase levels were increased at the end of the observation period following hypothermia without reaching statistical significance compared to normothermic groups. Liver function was preserved (p⩽ 0.05) after the rewarming period in hypothermic animals but showed no difference at the end of the observation period. In H&E staining, cell death was slightly increased hypothermic animals and caspase-3 staining displayed tendency towards more apoptosis in hypothermic group as well. CONCLUSIONS: Induction of hypothermia could not significantly improve hepatic damage during the first 48 h following major trauma. Further studies focusing on multi-organ failure including a longer observation period are required to illuminate the impact of hypothermia on hepatic function in multiple trauma patients.
Databáze: OpenAIRE
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