Degradation of human myelin phospholipids by phospholipas-enriched culture media of pathogenic Naegleria fowleri
| Autor: | Richard C. Franson, Robert M. Hysmith |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Biophysics
Phospholipase Biology Biochemistry Naegleria Substrate Specificity Microbiology chemistry.chemical_compound Endocrinology Meningoencephalitis Phosphatidylcholine parasitic diseases Animals Humans Myelin Sheath Phospholipids Phosphatidylethanolamine Naegleria fowleri Eukaryota Lysophosphatidylethanolamine biology.organism_classification Culture Media Kinetics Lysophosphatidylcholine chemistry Phospholipases lipids (amino acids peptides and proteins) Sphingomyelin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 712:698-701 |
| ISSN: | 0005-2760 |
| Popis: | Cell-free media from cultures of virulent Naegleria fowleri were tested for phospholipase activities and their ability to degrade phospholipids of human myelin. Virulent N. fowleri selectively released lipolytic enzymes into the media at various times during growth and hydrolyzed the phospholipids of human myelin, while media from virulent-attenuated and nonpathogenic Naegleria spp. were almost totally inactive. Hydrolysis of myelin phospholipid increased concomitantly with amebal growth, and the relative rate of breakdown at pH 7.5 was sphingomyelin greater than phosphatidylcholine greater than phosphatidylethanolamine. Elevated levels of lysophosphatidylcholine and lysophosphatidylethanolamine were also noted. |
| Databáze: | OpenAIRE |
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