T-Cell Receptor-Independent Activation of Clonal Th2 Cells Associated With Chronic Hypereosinophilia
Autor: | Michel Goldman, Liliane Schandené, Florence Roufosse, Elie Cogan, André Efira, B. Kennes, Catherine Sibille |
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Rok vydání: | 1999 |
Předmět: |
Adult
Interleukin 2 CD3 Complex CD3 medicine.medical_treatment Immunology Antigen presentation Receptors Antigen T-Cell Biology Lymphocyte Activation Biochemistry Th2 Cells Immune system Eosinophilia medicine Humans IL-2 receptor CD28 Cell Differentiation Cell Biology Hematology Dendritic cell Clone Cells Cytokine CD4 Antigens Cancer research biology.protein Cytokines Female medicine.drug |
Zdroj: | Scopus-Elsevier |
ISSN: | 1528-0020 0006-4971 |
DOI: | 10.1182/blood.v94.3.994.415k26_994_1002 |
Popis: | We recently observed a clonal expansion of CD3−CD4+ T cells secreting Th2-type cytokines in patients presenting chronic hypereosinophilia. As clonal T cells isolated from such patients did not spontaneously secrete cytokines in vitro, we reasoned that costimulatory signals delivered by antigen-presenting cells might be required to induce their full activation. To address this question, we investigated in two such patients the responses of CD3−CD4+ T cells to dendritic cells (DC). DC elicited proliferation and production of interleukin-5 (IL-5) and IL-13 by clonal cells from patient 1 and upregulated their expression of CD25 (IL-2R-). These effects were abolished when blocking monoclonal antibodies (MoAbs) against IL-2R- and IL-2 were added to cocultures, indicating critical involvement of an autocrine IL-2/IL-2R pathway. Cells from patient 2 were stimulated by DC to produce Th2 cytokines only when rIL-2 or rIL-15 was added to cocultures. In both patients, addition of inhibitory MoAbs against B7-1/B7-2 or CD2 to cocultures resulted in dramatic reduction of cytokine production and inhibited CD25 upregulation. Thus, TCR/CD3-independent activation of clonal Th2 cells by DC is an IL-2–dependent process, which requires signaling through CD2 and CD28. |
Databáze: | OpenAIRE |
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