Investigating CaOx Crystal Formation in Absence and Presence of Polyphenols under Microfluidic Conditions in Relation with Nephrolithiasis

Autor: Paszkál Papp, Karol Rakotozandriny, Dezső Horváth, Ali Abou-Hassan, Samantha Bourg, Ivan T. Lucas, Florence Babonneau, Christian Bonhomme, Ágota Tóth
Přispěvatelé: Spectroscopie, Modélisation, Interfaces pour L'Environnement et la Santé (LCMCP-SMiLES), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), PHysicochimie des Electrolytes et Nanosystèmes InterfaciauX (PHENIX), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), MTA-SZTE Department of Physical Chemistry and Materials Science, University of Szeged (MTA-SZTE), Laboratoire Interfaces et Systèmes Electrochimiques (LISE), National Research, Development and Innovation Office (NN125746), GINOP-2.3.2-15-2016-00013 project, doctoral school 397 'Physique et Chimie des Matériaux' (Sorbonne Université), ANR-11-IDEX-0004,SUPER,Sorbonne Universités à Paris pour l'Enseignement et la Recherche(2011)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Crystal Growth & Design
Crystal Growth & Design, American Chemical Society, 2020, pp.7683-7693. ⟨10.1021/acs.cgd.0c00929⟩
ISSN: 1528-7483
1528-7505
DOI: 10.1021/acs.cgd.0c00929⟩
Popis: International audience; Nephrolithiasis is a major health concern in western countries. Herein, we propose a microfluidic based approach to mimic the physical and physicochemical conditions encountered in the collecting duct in a nephron where calcium oxalate (CaOx) precipitation occurs. Our objective is to understand the parameters involved in the formation of such crystals. The microfluidic platform is reversible, allowing interfacial characterizations using scanning electron microscopy imaging and Raman spectroscopy. CaOx crystalline phases and morphologies were studied with respect to hydrodynamics and physicochemical conditions within the channel and at the outlet. While calcium oxalate monohydrate (COM) crystals were dominant within the channel, at the outlet, the crystals were calcium oxalate dihydrate (COD) crystals, which agrees with medical observations. Decreasing the flow rate lowered down the induction time for CaOx formation and enhanced the occurrence of COD crystals. The kinetics of COM crystals growth studied in situ showed two regimes, an initial surface-limited reaction, followed by a transport-limited growth with a dependency of the kinetics on the position of the crystal in the channel. Numerical modeling of CaOx formation in a microchannel using an in-house model considering the chemical reactions involved allowed to confirm the experimental observations on the location of precipitate formation but also to quantitatively match the scaling law related to the early growth of precipitate particles. Finally, the effect of polyphenols naturally found in green tea (GT) on modulating CaOx crystallization was studied in the microfluidic device in different scenarios where GT was initially mixed in solution with the Ca and/or the Ox precursors. The formation of COD crystals rather than COM ones was always predominant; however, depending on the conditions, CaOx crystals of different morphologies could be observed, including COD crystals with an elongated (100) crystalline face and COM crystals with a round-shaped morphology with a concave crystalline face.
Databáze: OpenAIRE
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