Artefact reduction for cell migration visualization using spectral domain optical coherence tomography
| Autor: | Boris Hermann, Gerald Matz, Kate Powell, Bernd Hofer, Sara Rey, Vedran Kajić, Alexandre R. Tumlinson, Boris Povazˇay, Wolfgang Drexler |
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| Rok vydání: | 2010 |
| Předmět: |
Retinal Ganglion Cells
Microscope Materials science General Physics and Astronomy Image processing 02 engineering and technology 01 natural sciences Retinal ganglion General Biochemistry Genetics and Molecular Biology law.invention Cell Line 010309 optics Optics Optical coherence tomography law Cell Movement 0103 physical sciences Microscopy medicine Image noise Image Processing Computer-Assisted General Materials Science Dictyostelium medicine.diagnostic_test business.industry General Engineering General Chemistry 021001 nanoscience & nanotechnology body regions sense organs Tomography 0210 nano-technology business Artifacts Tomography Optical Coherence Coherence (physics) |
| Zdroj: | Journal of Biophotonics; Vol 4 |
| ISSN: | 1864-0648 |
| Popis: | Visualization of cell migration during chemotaxis using spectral domain optical coherence tomography (OCT) requires non-standard processing techniques. Stripe artefacts and camera noise floor present in OCT data prevent detailed computer-assisted reconstruction and quantification of cell locomotion. Furthermore, imaging artefacts lead to unreliable results in automated texture based cell analysis. Here we characterize three pronounced artefacts that become visible when imaging sample structures with high dynamic range, e.g. cultured cells: (i) time-varying fixed-pattern noise; (ii) stripe artefacts generated by background estimation using tomogram averaging; (iii) image modulations due to spectral shaping. We evaluate techniques to minimize the above mentioned artefacts using an 800 nm optical coherence microscope. Effect of artefact reduction is shown exemplarily on two cell cultures, i.e. Dictyostelium on nitrocellulose substrate, and retinal ganglion cells (RGC-5) cultured on a glass coverslip. Retinal imaging also profits from the proposed processing techniques. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| Databáze: | OpenAIRE |
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