Multifunctional in vivo imaging of pancreatic islets during diabetes development
| Autor: | Meliza G. Ward, Binlin Wu, Mingming Hao, Sushmita Mukherjee, Ge Li, Angie C. N. Chong, Shuibing Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty endocrine system endocrine system diseases Anterior Chamber Carbohydrate metabolism Biology Diet High-Fat Clinical onset Fluorescence 03 medical and health sciences Islets of Langerhans Mice Imaging Three-Dimensional In vivo Computer Systems Internal medicine Diabetes mellitus medicine Tools and Techniques Diabetes Mellitus Animals Humans Cell Proliferation geography geography.geographical_feature_category Cell growth Pancreatic islets Macrophages Cell Biology Islet medicine.disease Cell biology Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Glucose Collagen Preclinical imaging Injections Intraperitoneal |
| Popis: | Pancreatic islet dysfunction leading to insufficient glucose-stimulated insulin secretion triggers the clinical onset of diabetes. How islet dysfunction develops is not well understood at the cellular level, partly due to the lack of approaches to study single islets longitudinally in vivo. Here we present a noninvasive, high-resolution system to quantitatively image real-time glucose metabolism from single islets in vivo, currently not available with any other method. In addition, this multifunctional system simultaneously reports islet function, proliferation, vasculature and macrophage infiltration in vivo from the same set of images. Applying our method to a longitudinal high fat diet study revealed changes in islet function as well as alternations in islet microenvironment. More importantly, this label-free system enabled us to image real-time glucose metabolism directly from single human islets in vivo for the first time, opening the door to noninvasive longitudinal in vivo studies of healthy and diabetic human islets. |
| Databáze: | OpenAIRE |
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