Pre- and post-prandial expression of genes involved in lipid metabolism at the end of the overfeeding period of mule ducks

Autor: Bernadet Marie-Dominique, Davail Stéphane, Gontier Karine, Tavernier Annabelle, Ricaud Karine
Přispěvatelé: Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Palmipèdes à Foie Gras (UEPFG), Institut National de la Recherche Agronomique (INRA), We thank the ‘‘Conseil Général des Landes’’ and the ‘‘Comité Interprofessionnel des Palmipèdes à Foie Gras’’ (CIFOG) for financing this work.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_specialty
duck
gavage
Clinical Biochemistry
kinetic
aliment
Adipose tissue
030204 cardiovascular system & hematology
canard mulard
Avian Proteins
liver steatosis
03 medical and health sciences
0302 clinical medicine
stéatose hépatique
Internal medicine
lipid metabolism
force feeding
medicine
Animals
foie gras
Molecular Biology
Lipoprotein lipase
métabolisme lipidique
biology
hepatic steatosis
[SDV.BA]Life Sciences [q-bio]/Animal biology
Lipid metabolism
Cell Biology
General Medicine
Postprandial Period
medicine.disease
foodstuff
Fatty acid synthase
Ducks
030104 developmental biology
Postprandial
Endocrinology
Gene Expression Regulation
Liver
LDL receptor
biology.protein
lipids (amino acids
peptides
and proteins)
Steatosis
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Lipoprotein
expression des gènes
métabolisme postprandial
Zdroj: Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry, Springer Verlag, 2018, 438 (1-2), pp.111-121. ⟨10.1007/s11010-017-3118-6⟩
BASE-Bielefeld Academic Search Engine
ISSN: 0300-8177
1573-4919
DOI: 10.1007/s11010-017-3118-6⟩
Popis: In palmipeds, overfeeding leads to hepatic steatosis, also called "foie gras" which is the result of many metabolic mechanisms. In order to understand these mechanisms, we decided to measure the expression of genes implicated in lipid metabolism during 12 hours (h) following the last meal of the overfeeding period. We have shown that there is a precocious expression (within 2 h) of fatty acid synthase and acyl CoA synthetase long-chain 1 in liver and muscle of mule ducks in addition with a later peak. Furthermore, di-acyl glycerol acyl transferase presents the highest induction of expression in liver and it is overexpressed quite a long time, positioning this enzyme as a key factor in hepatic steatosis. These observations are quite similar in muscle. Lipoprotein secretion is upregulated later in postprandial period, with an upregulation of apolipoprotein and microsomal triglycerides transfer protein beginning at 5 h in liver or muscle. Regarding hepatic re-uptake of lipid, lesser variations are observed, suggesting that fatty acid binding protein and very low-density lipoprotein receptor (VLDLR) are already at their maximum expression specifically in these tissues. In muscle, VLDLR and LDLR upregulation is observed 5 h after the meal, associated with an overexpression in the adipose tissue of lipase maturation factor 1 involved in the maturation of lipoprotein lipase. These findings will allow us to better understand the kinetic treatment in lipid metabolism after a meal in overfed ducks. This first report on kinetic expression will allow researcher to better target their sampling time knowing the optimal point of expression of each gene.
Databáze: OpenAIRE
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