WT1 overexpression: A clinically useful marker in acute and chronic myeloid leukemias
| Autor: | Giuseppe Saglio, Francesca Arruga, Sara Capella, Anna Marina Liberati, Daniela Cilloni, Maria Rauco, Ilaria Defilippi, Sara Grillo, Sonia Carturan, Valentina Rosso |
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| Rok vydání: | 2005 |
| Předmět: |
EXPRESSION
Genes Wilms Tumor TUMOR GENE WT1 MINIMAL RESIDUAL DISEASE Biology Fusion gene Predictive Value of Tests Recurrence medicine Humans Regulation of gene expression Acute leukemia Myelodysplastic syndromes Hematology Gene rearrangement medicine.disease Minimal residual disease Gene Expression Regulation Neoplastic Haematopoiesis Leukemia PCR Leukemia Myeloid Acute Disease Chronic Disease CELLS Immunology Cancer research Biomarkers |
| Zdroj: | Hematology. 10:76-78 |
| ISSN: | 1607-8454 |
| DOI: | 10.1080/10245330512331390005 |
| Popis: | Monitoring of acute leukemia patients during and after treatment for the presence of remaining leukemic cells minimal residual disease (MRD) have been shown to give major insight into the effectiveness of treatment. However, so far applicability of this strategy has been limited to those leukaemia subsets characterized by genetic markers amenable to sensitive detection by PCR. Although PCR for immunoglobulin and T-cell receptor gene rearrangement represents the gold standard for MRD detection in most cases of acute lymphoblastic leukemias (ALL) lacking the availability of fusion gene transcripts as molecular markers, the situation in AML is more complicated because, at present, more than 50% of them lack any sort of clonality markers suitable for MRD monitoring. Thus, a number of studies have been performed in the attempt to identify cytogenetic and molecular abnormalities associated with leukemic transformation. The Wilms Tumor Gene (WT1) represents a molecular marker for the detection of the leukemic clone useful for monitoring the presence of leukemic cells in all the patients affected by acute and chronic leukemias as well as myelodysplastic syndromes. The WT1 gene, cloned in 1990 by Call et al. [1] encodes for a protein with the characteristics of a zinc finger transcription factor. WT1 expression is restricted to a small number of tissues [2] including testis, ovaries, myometrium, stromal cells of the uterus, heart, lung, intestine, liver and in the supportive stroma and splenic capsule of the spleen [2]. In contrast, several other tissues and cell lines were negative for WT1 expression. Although the role of the WT1 gene in the development of malignancies in the kidney appears quite well defined, currently its potential function in human hematopoiesis still needs to be clarified. The role of WT1 in the leukemogenesis process appears |
| Databáze: | OpenAIRE |
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