Convenient detection of E. coli in Ringer's solution
| Autor: | Thomas Bocklitz, Susanne Pahlow, Karina Weber, Dana Cialla, Martha Schwarz, Carolin Steinbrücker, Jürgen Popp |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Silver Chromatography Chemistry DNA–DNA hybridization Analytical chemistry Metal Nanoparticles Polymerase Chain Reaction Biochemistry Silver nanoparticle Ringer's Solution Analytical Chemistry genomic DNA Complementary DNA Escherichia coli Electrochemistry Environmental Chemistry Ringer's solution Naked eye Isotonic Solutions DNA Probes Spectroscopy |
| Zdroj: | The Analyst. 138:5866 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c3an01240a |
| Popis: | An easy and inexpensive detection method for DNA hybridization assays combining magnetic beads and enzymatically generated silver nanoparticles is introduced. The main advantage of this approach is the possibility to distinguish between positive and negative test results with the naked eye. In the case of complementary DNA sequences the sample will turn black within a few minutes, allowing readout without any hardware. In order to illustrate the applicability of the assay genomic DNA isolated from E. coli contaminated Ringer's solution was used for testing the sensitivity as well as specificity. |
| Databáze: | OpenAIRE |
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