Fingerprinting the macro-organisation of pigment-protein complexes in plant thylakoid membranes in vivo by circular-dichroism spectroscopy
| Autor: | Herbert van Amerongen, Neha Rai, Katalin Solymosi, Győző Garab, Tünde N. Tóth, László Kovács, Wolfgang P. Schröder, Ottó Zsiros, Peter Horton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Circular dichroism Chloroplasts Light-Harvesting Protein Complexes Biophysics Xanthophylls Biology Photosynthesis Thylakoids 01 natural sciences Biochemistry Thylakoid membrane Psi-type CD Light-harvesting complex 03 medical and health sciences Pigment In vivo Chiral macrodomain Photosystem II Protein Complex food and beverages Cell Biology Plant Leaves 030104 developmental biology Biofysica Thylakoid visual_art visual_art.visual_art_medium Light-harvesting complexes Photosystem II supercomplexes EPS 010606 plant biology & botany |
| Zdroj: | Biochimica et Biophysica Acta. B, Bioenergetics, 1857(9), 1479-1489 Biochimica et Biophysica Acta. B, Bioenergetics 1857 (2016) 9 |
| ISSN: | 0005-2728 |
| Popis: | Macro-organisation of the protein complexes in plant thylakoid membranes plays important roles in the regulation and fine-tuning of photosynthetic activity. These delicate structures might, however, undergo substantial changes during isolating the thylakoid membranes or during sample preparations, e.g., for electron microscopy. Circular-dichroism (CD) spectroscopy is a non-invasive technique which can thus be used on intact samples. Via excitonic and psi-type CD bands, respectively, it carries information on short-range excitonic pigment-pigment interactions and the macro-organisation (chiral macrodomains) of pigment-protein complexes (psi, polymer or salt-induced). In order to obtain more specific information on the origin of the major psi-type CD bands, at around (+)506, (-)674 and (+)690 nm, we fingerprinted detached leaves and isolated thylakoid membranes of wild-type and mutant plants and also tested the effects of different environmental conditions in vivo. We show that (i) the chiral macrodomains disassemble upon mild detergent treatments, but not after crosslinking the protein complexes; (ii) in different wild-type leaves of dicotyledonous and monocotyledonous angiosperms the CD features are quite robust, displaying very similar excitonic and psi-type bands, suggesting similar protein composition and (macro-) organisation of photosystem II (PSII) supercomplexes in the grana; (iii) the main positive psi-type bands depend on light-harvesting protein II contents of the membranes; (iv) the (+)506 nm band appears only in the presence of PSII-LHCII supercomplexes and does not depend on the xanthophyll composition of the membranes. Hence, CD spectroscopy can be used to detect different macro-domains in the thylakoid membranes with different outer antenna compositions in vivo. |
| Databáze: | OpenAIRE |
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