A sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein A-V concentration
| Autor: | Norman E. Miller, Takeshi Kujiraoka, Masahiro Tsuji, Tadao Iwasaki, Mayumi Takano, Mitsuaki Ishihara, Hiroaki Hattori, Makoto Nagano, Hajime Ide, Jun Ishii, Irina P. Miller |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Apolipoprotein E
Male medicine.medical_specialty Apolipoprotein B Enzyme-Linked Immunosorbent Assay QD415-436 Apolipoproteins A Biochemistry Polymorphism Single Nucleotide chemistry.chemical_compound Endocrinology Apolipoproteins E Polymorphism (computer science) single nucleotide polymorphism Reference Values Internal medicine medicine Humans triglyceride immunoassay Triglycerides Sex Characteristics medicine.diagnostic_test Triglyceride biology Apolipoprotein A-I Cholesterol Cholesterol HDL nutritional and metabolic diseases Antibodies Monoclonal Cell Biology Apolipoproteins chemistry Diabetes Mellitus Type 2 Apolipoprotein A-V Immunoassay diabetes mellitus biology.protein lipids (amino acids peptides and proteins) Female Antibody |
| Zdroj: | Journal of Lipid Research, Vol 46, Iss 9, Pp 2015-2022 (2005) |
| ISSN: | 0022-2275 |
| Popis: | Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106 patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V concentration was 179.2 +/- 74.8 ng/ml, and it was greater in females than in males (P < 0.005). It was correlated positively with the plasma HDL cholesterol (r = 0.32, P < 0.0001), apoA-I (r = 0.27, P = 0.0001), and apoE (r = 0.18, P = 0.011) concentrations and negatively with plasma TG concentration (r = -0.22, P = 0.021). In relation to single nucleotide polymorphism 3 (-1131C/T) of the apoA-V gene, apoA-V concentration was higher in the T/T type than in the C/C type (P < 0.01). Plasma TG concentration was lower in the T/T type than in the C/C or C/T type (P < 0.05). ApoA-V concentration was lower in the diabetic patients (69.4 +/- 44.3 ng/ml; P < 0.01) than in the healthy controls. |
| Databáze: | OpenAIRE |
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