Peripheral blood and intrathyroidal T cell clones from patients with thyroid autoimmune diseases
| Autor: | G. Caroff, D. Maugendre, N. Genetet, J. Gibassier, C Massart |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cytotoxicity
Immunologic endocrine system Pathology medicine.medical_specialty Cytoplasm endocrine system diseases medicine.medical_treatment T cell Graves' disease T-Lymphocytes Immunology Thyroid Gland Thyroiditis medicine Immunology and Allergy Cytotoxic T cell Humans Interferon gamma Cells Cultured Autoimmune disease business.industry Tumor Necrosis Factor-alpha Thyroid Thyroiditis Autoimmune medicine.disease Graves Disease Cytokine medicine.anatomical_structure Phenotype Antigens Surface Leukocytes Mononuclear Cytokines business Cell Division medicine.drug |
| Zdroj: | Autoimmunity. 31(3) |
| ISSN: | 0891-6934 |
| Popis: | For a better understanding of the pathogenesis of thyroid autoimmune diseases, we have studied morphological and functional properties of T clones from peripheral blood lymphocytes (PBL) and from intrathyroidal lymphocytes (ITL) obtained from 3 patients with Graves' disease or 1 Hashimoto's thyroiditis. Investigations were carried out on clones cultured alone or cocultured with autologous thyrocytes. Clonage efficiency ranged from 30% to 33% for PBL and 10% to 36% for ITL. A predominance of CD4-positive clones was observed whatever the origin of the lymphocytes or the autoimmune pathology. Gamma interferon (IFN-gamma) was detected in the majority (17/19) of the clones tested. Intracytoplasmic interleukin (IL-4) was secreted in 7/19 clones and both cytokines were produced in 5/19 clones. In coculture a proliferative response and tumour necrosis factor (TNF-alpha) production were observed with 6 clones (4 from Graves thyrocytes and 2 from thyroiditis). No cytotoxic clone was derived from Graves or thyroiditis tissues. These data demonstrate that the large majority of T clones are principally CD4-T cells; all the clones secreted TNF-alpha and a large majority produced IFN-gamma. Only a few clones produced IL-4 alone or associated with IFN-gamma. Six T clones induced proliferative response and of TNF-alpha secretion in coculture. Further investigations must be performed on these antigen-reactive T clones to analyse their role in the pathogenesis of the human thyroid autoimmune diseases. |
| Databáze: | OpenAIRE |
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