Proliferation (Ki-67 and Phosphohistone H3) and Oncotype DX Recurrence Score in Estrogen Receptor-positive Breast Cancer
| Autor: | Amy L. Adams, Andrew J. Page, Beth Chastain, Daron J. Williams, Mary Darrow, Cynthia Cohen |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Oncology
medicine.medical_specialty Histology medicine.drug_class Estrogen receptor Breast Neoplasms Pathology and Forensic Medicine Histones Breast cancer Predictive Value of Tests Recurrence Internal medicine Biomarkers Tumor medicine Humans Cells Cultured Cell Proliferation Image Cytometry Staining and Labeling biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Prognosis medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Medical Laboratory Technology Ki-67 Antigen Receptors Estrogen Estrogen Ki-67 biology.protein Female Lymph Antibody business Oncotype DX |
| Zdroj: | Applied Immunohistochemistry & Molecular Morphology. 19:431-436 |
| ISSN: | 1541-2016 |
| DOI: | 10.1097/pai.0b013e318206d23d |
| Popis: | The Oncotype DX Recurrence Score (RS) is often used in lymph node-negative, estrogen receptor-positive breast cancer to refine prognosis and direct therapy. Its utility is limited by its cost, proprietary nature, and turnaround time. Markers of proliferation factor heavily into determination of RS. Our aim is to correlate expression of proliferation markers Ki-67 and phosphohistone H3 (PPH3) with RS and other prognostic indicators. Estrogen receptor-positive invasive breast carcinomas from 133 patients with Oncotype DX testing were selected. Representative tumor sections were stained with MIB1, a monoclonal antibody that reacts against Ki-67, and antibody to PPH3. Nuclear staining was quantitated through an automated imaging system. The percentage of positive cells was scored as low (10%), intermediate (10% to 20%), or high (20%) for Ki-67, and low (2%), intermediate (2% to 5%), or high (5%) for PPH3. Expression of both markers was compared with RS and clinicopathologic parameters including grade, tumor size, lymph node metastasis, and angiolymphatic invasion. Ki-67 and PPH3 expression were both significantly associated with RS (P=0.02 and P=0.027, respectively) and grade (P0.001 and P=0.002, respectively). Ki-67 expression correlated with angiolymphatic invasion (P=0.01) but not with tumor size or lymph node metastasis; PPH3 expression showed no association with any of these 3 parameters. Expression of proliferation markers Ki-67 and PPH3 by immunohistochemistry is significantly correlated with RS and tumor grade. This observation suggests that immunohistochemical assessment of markers of proliferation may provide useful prognostic information, at lower cost than RS testing. |
| Databáze: | OpenAIRE |
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