Determination of (13C) urea enrichment by gas chromatography/mass spectrometry and gas chromatography/isotope ratio mass spectrometry
| Autor: | Michel Beylot, France David, Yadh Khalfallah, Sylvie Normand, Valerie Large, Henri Brunengraber |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Carbon Isotopes Chromatography Isotope Chemistry Substrate (chemistry) Mass spectrometry Biochemistry Sample preparation in mass spectrometry Gas Chromatography-Mass Spectrometry Rats Rats Sprague-Dawley chemistry.chemical_compound Urea Lactates Molecular Medicine Animals Humans Gas chromatography Lactic Acid Gas chromatography–mass spectrometry Isotope-ratio mass spectrometry Spectroscopy |
| Zdroj: | Biological mass spectrometry. 23(8) |
| ISSN: | 1052-9306 |
| Popis: | We present gas chromatographic/mass spectrometric and gas chromatographic/isotope ratio mass spectrometric assays of the 13C enrichment of plasma urea converted to its dimethylaminomethylene derivative. The limits of sensitivity of the two techniques are 0.2% and 0.02%, respectively. The techniques were tested in rats and humans infused with (13C)urea or (3-13C)lactate. (13C)Urea enrichment during the infusion of (3-13 C)lactate in humans was not detectable by gas chromatography/mass spectrometry but was easily measured by gas chromatography/isotope ratio mass spectrometry. These assays should be useful for clinical investigations, in which the incorporation of a (13C)gluconeogenic substrate into glucose must be corrected for the incorporation of 13CO2 derived from the oxidation of the substrate. This correction involves measuring the low-level 13C enrichment of urea. |
| Databáze: | OpenAIRE |
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