Isolation and purification of a fibrinogenolysin from the venom of the saw-scaled viper (Echis carinatus) by high-performance liquid chromatography
| Autor: | A. Tseng, S.L. Berry, B. Cotton, T. Exner, D.D. Sheumack, M.E.H. Howden |
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| Rok vydání: | 1989 |
| Předmět: |
Chromatography
biology Chemistry Organic Chemistry Venom Biological activity Viper Venoms General Medicine Fractionation Saw-scaled viper biology.organism_classification complex mixtures Biochemistry High-performance liquid chromatography Analytical Chemistry Hydrophobic effect Echis carinatus Fibrinolytic Agents Spectrophotometry Ultraviolet Amino Acids Amino acid residue Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 474:424-429 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)93938-x |
| Popis: | This paper describes the successful fractionation of crude snake venoms by hydrophobic interaction HPLC, with retention of biological activity. A fibrinogenolysin, with its biological activity intact, was isolated from crude E. carinatus venom with use of a hydrophobic interaction column (TSK-Phenyl 5PW). After further purification by ion-exchange and reserved-phase HPLC, fourteen amino acid residues of its N-terminal sequence were determined |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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