Subtyping of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Makkah, Saudi Arabia
| Autor: | Saeed A. Al-Harthi, Mohammed A. El-Bali, Rowaida A. Bakri, Mona A. Abdel-Fatah, Mowafy Nm, Raafat T. Mohamed, Mohamed El-Malky, Dina A. Zaghlool, Anhar A. Mohamed |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Veterinary medicine Blastocystis sp 030231 tropical medicine Saudi Arabia Blastocystis Infections DNA Ribosomal Asymptomatic Gastroenterology lcsh:Infectious and parasitic diseases Sequence-Tagged Sites (STS) PCR Feces Subtyping 03 medical and health sciences 0302 clinical medicine Internal medicine Epidemiology medicine Humans lcsh:RC109-216 Asymptomatic Infections Blastocystis biology Research DNA Protozoan Middle Aged 030108 mycology & parasitology Ribosomal RNA biology.organism_classification Infectious Diseases Parasitology Makkah city Tropical medicine Female medicine.symptom |
| Zdroj: | Parasites & Vectors, Vol 10, Iss 1, Pp 1-7 (2017) Parasites & Vectors |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-017-2114-8 |
| Popis: | Background Blastocystis is a group of cosmopolitan gastrointestinal parasite of humans and a wide variety of animals. These anaerobic protozoans include more than 17 specific small-subunit ribosomal RNA subtypes, of which nine are found in humans with a variable geographical distribution. Until now, no study has described the Blastocystis subtypes present in Saudi Arabia. Methods In total, 1,262 faecal samples were collected from patients with gastrointestinal complaints and asymptomatic individuals visiting two major hospitals. All samples were analysed by F1/R1 diagnostic PCR, microscopy and culture methods. The subtypes of Blastocystis sp. isolates were determined by the sequenced-tagged site (STS)-based method. Results One-hundred-thirty-three positive cases were detected by F1/R1 diagnostic PCR, of which 122 were also positive by the culture method and 83 by direct microscopy. The sensitivities of direct microscopy and the culture method were 62% and 92%, respectively. Subtype (ST3) was the most prevalent (80.5%), followed by ST1 (14.5%) and ST2 (5%). ST4, ST5, ST6 and ST7 were not detected in this study. ST3 infections were significantly predominant (P |
| Databáze: | OpenAIRE |
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