Functional polymorphism of cyclooxygenase-2 gene (G–765C) in chronic obstructive pulmonary disease patients
Autor: | Maciej Banasiak, Andrzej Witusik, Michał Panek, Janusz Szemraj, Paweł Górski, Adam Antczak, Tadeusz Pietras |
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Rok vydání: | 2011 |
Předmět: |
Male
medicine.medical_specialty Genotype Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Gastroenterology Pulmonary Disease Chronic Obstructive Gene Frequency Risk Factors Internal medicine Odds Ratio Genetics medicine Humans Genetic Predisposition to Disease Allele Promoter Regions Genetic Molecular Biology Allele frequency Genotyping Genetic Association Studies COPD General Medicine Odds ratio medicine.disease Cyclooxygenase 2 Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
Zdroj: | Molecular Biology Reports. 39:2163-2167 |
ISSN: | 1573-4978 0301-4851 |
Popis: | Cyclooxygenase two (COX-2) is an important enzyme metabolizing arachidonic acid. In contrast to constitutive cyclooxygenase one (COX-1), COX-2 is induced by proinflammatory factors. Polymorphism -765 G/C in COX-2-encoding gene promoter is associated with development of Alzheimer's disease, depression, carcinoma of the pancreas in smokers, breast cancer and rheumatoid arthritis. It is interesting whether the -765 G/C polymorphism in COX-2-encoding gene promoter can be associated with COPD, a disease which is inflammatory in character. It is highly probable as the breast and pancreas cancers, whose associations with the analyzed polymorphism have been studied, are smoking-dependent tumors. Additionally, tobacco smoke has been demonstrated to induce COX-2 in the lungs. The study group consisted of 122 COPD patients (48 females, 74 males). The control group consisted of 149 healthy nonsmoking subjects (83 females, 66 males). Polymerase chain reaction/restriction fragment length polymorphism was used for genotyping. A statistically significant difference in genotype distribution was observed as a result of the comparison between healthy subjects and patients with COPD. The distribution of alleles in both groups conformed with Hardy-Weinberg equilibrium. In the group of COPD patients, GG allele was found in 79 subjects, GC in 36, and CC in 7 subjects (F = 0.094, P = 0.296927); in the control group, 73 subjects had GG allele, 68--GC and 8--CC (F = 0.12728, P = 0.120265). The allele frequency revealed differences between those groups, attaining the level of statistical significance (χ(2) = 29.043, df = 2, P = 0.0000. The carriers of -765 G allele are at 1.53-fold higher risk of developing COPD. The presence of GG genotype does not increase significantly the risk of the disease. It is also noteworthy that the carriers of CC or GC genotypes are at significantly lower risk of developing COPD than the group of subjects with GG genotype. |
Databáze: | OpenAIRE |
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