Expression of apoptotic factors in vaginal tissues from women with urogenital prolapse
| Autor: | Jason Yen-Ping Ho, Bertha Chen, Yan Wen, Mary Lake Polan |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Urology Blotting Western bcl-X Protein Apoptosis Real-Time Polymerase Chain Reaction Severity of Illness Index Article California Pelvic Organ Prolapse Andrology Bcl-2-associated X protein Western blot Gene expression medicine Humans RNA Messenger bcl-2-Associated X Protein Regulation of gene expression biology medicine.diagnostic_test Genitourinary system business.industry Middle Aged medicine.anatomical_structure Real-time polymerase chain reaction Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 Case-Control Studies Vagina biology.protein Female bcl-Associated Death Protein Neurology (clinical) business |
| Zdroj: | Neurourology and Urodynamics. 30:1627-1632 |
| ISSN: | 0733-2467 |
| Popis: | Aims Increased apoptotic activity in pelvic tissues may contribute to development of pelvic floor disorders. We evaluated expression of apoptotic factors (Bcl-2 family) in vaginal tissues from women with pelvic organ prolapse (POP) and how these factors correlate with severity of prolapse. Methods mRNA and protein expression of anti-apoptotic and pro-apoptotic factors in vaginal tissues from subjects and controls were determined by real-time PCR and Western blot. Severity of prolapse was staged using POP-Q criteria. Results Differential expression of Bcl-2 family factors was observed in protein rather than in gene expression. During the secretory phase, the anti-apoptotic (Bcl-2, Bcl-xl) and pro-apoptotic protein (Bax) were upregulated in controls compared to cases (P |
| Databáze: | OpenAIRE |
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