Cytocompatibility, gene-expression profiling, apoptotic, mechanical and (29)Si, (31)P solid-state nuclear magnetic resonance studies following treatment with a bioglass-chitosan composite
| Autor: | Jebahi Samira, Rebai Treq, Pascal Pellen, Keskes Hassib, Oudadesse Hassane, Kallabi Fakhri, Elfeki Abdelfatteh, Saoudi Mongi |
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| Přispěvatelé: | Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) |
| Rok vydání: | 2014 |
| Předmět: |
Ceramics
Magnetic Resonance Spectroscopy Cell Survival Osteocalcin Bioengineering Apoptosis Core Binding Factor Alpha 1 Subunit 02 engineering and technology Bone tissue Applied Microbiology and Biotechnology law.invention 03 medical and health sciences law In vivo medicine [CHIM]Chemical Sciences Animals Humans Rats Wistar Bone regeneration ComputingMilieux_MISCELLANEOUS Cells Cultured 030304 developmental biology 0303 health sciences Chitosan biology Chemistry Gene Expression Profiling Osteoblast General Medicine 021001 nanoscience & nanotechnology Molecular biology 3. Good health RUNX2 medicine.anatomical_structure Bioactive glass Bone Substitutes Ovariectomized rat biology.protein 0210 nano-technology Biotechnology |
| Zdroj: | Biotechnology Letters Biotechnology Letters, 2014, 36 (12), pp.2571--2579. ⟨10.1007/s10529-014-1633-z⟩ Biotechnology Letters, Springer Verlag, 2014, 36 (12), pp.2571--2579. ⟨10.1007/s10529-014-1633-z⟩ |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-014-1633-z⟩ |
| Popis: | The performance therapy of chitosan (CH)-doped bioactive glass (BG) has been evaluated in vitro and in vivo. In vitro, the effect of CH-BG was assessed on human Saos-2 osteoblast cells. In vivo, Wistar rats were ovariectomized (OVX) and CH, BG and CH-BG were implanted in bone tissue. After 3 days of CH-BG contact, cell viability of Saos-2 osteoblast increased by 16.4% as compared to the control group. The runt-related transcription factor 2 (RUNX2/Cbfa1) and osteocalcin (OC) gene expressions were significantly increased with 600 and 300%, respectively, in contact of CH-BG as compared with CH. In vivo, the apoptotic index in the OVX-CH-BG group was decreased by 80%. A mechanical hardness test showed a significant bone strength improvement after CH-BG implantation (40%). The CH-BG composite may therefore prove clinically useful as a bioactive bone substitute. |
| Databáze: | OpenAIRE |
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