Oxidized LDL Modify the Human Adipocyte Phenotype to an Insulin Resistant, Proinflamatory and Proapoptotic Profile
| Autor: | Lourdes Garrido-Sánchez, Jose Rivas-Becerra, Flores Martín-Reyes, Luis Ocaña-Wilhelmi, Monica Tome, Francisco J. Tinahones, Franz Tatzber, Concepcion Santiago-Fernandez, Edith Pursch, Eduardo García-Fuentes |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Programmed cell death Glucose uptake lcsh:QR1-502 Adipose tissue 030204 cardiovascular system & hematology adipocyte Biochemistry Article lcsh:Microbiology Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipocyte Internal medicine Adipocytes Autophagy medicine Humans Adiponectin secretion Scavenger receptor Molecular Biology Inflammation oxidized low-density lipoprotein Esterification scavenger receptors apoptosis Biological Transport Cell Differentiation Lipoproteins LDL Cholesterol Phenotype 030104 developmental biology Endocrinology chemistry inflammation Female lipids (amino acids peptides and proteins) Insulin Resistance Biomarkers |
| Zdroj: | Biomolecules, Vol 10, Iss 534, p 534 (2020) Biomolecules Volume 10 Issue 4 |
| Popis: | Little information exists in humans on the regulation that oxidized low-density lipoprotein (oxLDL) exerts on adipocyte metabolism, which is associated with obesity and type 2 diabetes. The aim was to analyze the oxLDL effects on adipocytokine secretion and scavenger receptors (SRs) and cell death markers in human visceral adipocytes. Human differentiated adipocytes from visceral adipose tissue from non-obese and morbidly obese subjects were incubated with increasing oxLDL concentrations. mRNA expression of SRs, markers of apoptosis and autophagy, secretion of adipocytokines, and glucose uptake were analyzed. In non-obese and in morbidly obese subjects, oxLDL produced a decrease in insulin-induced glucose uptake, a significant dose-dependent increase in tumor necrosis factor-&alpha (TNF-&alpha ), IL-6, and adiponectin secretion, and a decrease in leptin secretion. OxLDL produced a significant increase of Lox-1 and a decrease in Cxcl16 and Cl-p1 expression. The expression of Bnip3 (marker of apoptosis, necrosis and autophagy) was significantly increased and Bcl2 (antiapoptotic marker) was decreased. OxLDL could sensitize adipocytes to a lower insulin-induced glucose uptake, a more proinflammatory phenotype, and could modify the gene expression involved in apoptosis, autophagy, necrosis, and mitophagy. OxLDL can upregulate Lox-1, and this could lead to a possible amplification of proinflammatory and proapoptotic effects of oxLDL. |
| Databáze: | OpenAIRE |
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