Tissue transglutaminase_variant 2‐transduced mesenchymal stem cells and their chondrogenic potential
| Autor: | Ayse Hande Nayman, Gamze Torun Kose, Dilek Telci, Ayse Ceren Calikoglu Koyuncu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cartilage
Articular Male 0106 biological sciences 0301 basic medicine Tissue transglutaminase Bioengineering Articular cartilage 01 natural sciences Applied Microbiology and Biotechnology Cartilage tissue engineering Rats Sprague-Dawley 03 medical and health sciences GTP-Binding Proteins Transduction Genetic 010608 biotechnology Poly-L-lactide medicine Animals Humans Regeneration Protein Glutamine gamma Glutamyltransferase 2 Cells Cultured Transglutaminases Tissue Engineering Tissue Scaffolds biology Chemistry Cartilage Mesenchymal stem cell Mesenchymal Stem Cells Chondrogenesis Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein Bone marrow Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 117:1839-1852 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.27311 |
| Popis: | As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) in mesenchymal stem cells (MSC) derived from bone marrow of rats. For this purpose, rat MSCs transduced with TGM2_v2 were grown and differentiated on three-dimensional polybutylene succinate (PBSu) and poly-l-lactide (PLLA) blend scaffolds. The transduced cells could not only successfully express the short form transglutaminase-2, but also deposited the protein onto the scaffolds. In addition, they could spontaneously produce cartilage-specific proteins without any chondrogenic induction, suggesting that TGM2_v2 expression provided the cells the ability of chondrogenic differentiation. PBSu:PLLA scaffolds loaded with TGM2_v2 expressing MSCs could be used in repair of articular cartilage defects. |
| Databáze: | OpenAIRE |
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