A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
| Autor: | Julián Fernández, Bruno Lomonte, Daniela Solano, Adolfo Rafael de Roodt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Snake venom Myotoxin Population Zoology Venom Toxicology medicine.disease_cause complex mixtures 03 medical and health sciences Phospholipase A2 medicine Lys49 Bothrops education education.field_of_study 030102 biochemistry & molecular biology biology Toxin biology.organism_classification Bothrops cotiara 030104 developmental biology biology.protein lipids (amino acids peptides and proteins) 615.946 Venenos animales |
| Zdroj: | Toxicon, vol. 148, pp. 143-148 Kérwá Universidad de Costa Rica instacron:UCR |
| Popis: | Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of Misiones has received little attention. While exploring the chromatographic venom profile of Argentinean B. cotiara, a major protein peak was found which, according to a previous study, is not present in the venom of Brazilian origin. The corresponding protein was isolated by RP-HPLC, and characterized by electrophoresis, mass spectrometry, phospholipase A2 (PLA2) assay, and myotoxic activities. Representing nearly 15% of B. cotiara venom from Misiones, this protein was identified as a Lys49 PLA2 homologue. In accordance with the characteristics of this toxin family, the protein induced myotoxicity in mice and was devoid of PLA2 activity. Since previous work reported that no PLA2 or PLA2- homologues occur in B. cotiara venom of Brazilian origin, the presence of an abundant Lys49 PLA2 homologue in the venom from Misiones highlights a striking phenotypic variation in toxin expression within two populations of a single snake species inhabiting different geographic areas. The considerable proportion of B. cotiara Lys49 PLA2 homologue myotoxin in the venom alerts that skeletal muscle necrosis might be a potentially relevant consequence of eventual envenomings by this species in Misiones. Universidad de Costa Rica/[741-B5-602]/UCR/Costa Rica UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|