Metabolic adaptations to HFHS overfeeding: how whole body and tissues postprandial metabolic flexibility adapt in Yucatan mini-pigs

Autor: Annick Bernalier-Donadille, Dominique Dardevet, Sergio Polakof, Estelle Pujos-Guillot, Blandine Comte, Isabelle Savary-Auzeloux, Didier Rémond, Mathieu Rambeau
Přispěvatelé: Unité de Nutrition Humaine (UNH), Clermont Université-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Recherche Agronomique (INRA), Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])
Rok vydání: 2016
Předmět:
Blood Glucose
0301 basic medicine
Swine
Transamination
medicine.medical_treatment
Medicine (miscellaneous)
Adipose tissue
postprandial
régime riche en saccharose
Urine
métabolisme des lipides
0302 clinical medicine
Dietary Sucrose
Homeostasis
Insulin
Amino Acids
Phosphorylation
2. Zero hunger
chemistry.chemical_classification
Nutrition and Dietetics
glyceride metabolism
High-fat–high-sucrose diet
Postprandial Period
Adaptation
Physiological
Cholesterol
Postprandial
Liver
mini-pig
mini porc
Lipogenesis
Swine
Miniature
Female
Leucine
aliment riche en graisse
métabolomique
metabolomic
medicine.medical_specialty
030209 endocrinology & metabolism
Hyperphagia
Biology
Diet
High-Fat
glucose and lipid metabolism
03 medical and health sciences
Internal medicine
Metabolome
medicine
Animals
Metabolomics
métabolisme du glucose
Triglycerides
Catabolism
Gluconeogenesis
Lipid Metabolism
Glucose
030104 developmental biology
Endocrinology
chemistry
Energy Metabolism
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: European Journal of Nutrition
European Journal of Nutrition, Springer Verlag, 2016, [Epub ahead of print, pp.1-17. ⟨10.1007/s00394-016-1302-1⟩
European Journal of Nutrition, 2018, 57 (1), pp.119-135. ⟨10.1007/s00394-016-1302-1⟩
European Journal of Nutrition, Springer Verlag, 2018, 57 (1), pp.119-135. ⟨10.1007/s00394-016-1302-1⟩
ISSN: 1436-6215
1436-6207
DOI: 10.1007/s00394-016-1302-1
Popis: The authors acknowledge J. David, C. Prolhac, D. Durand and the personnel of the Animal Facility (C. de L’Homme, B. Cohade) for technical assistance; PurposeIn the present study, we aimed to metabolically characterize the postprandial adaptations of the major tissues involved in energy, lipids and amino acids metabolisms in mini-pigs.MethodMini-pigs were fed on high-fat–high-sucrose (HFHS) diet for 2 months and several tissues explored for metabolic analyses. Further, the urine metabolome was followed over the time to picture the metabolic adaptations occurring at the whole body level following overfeeding.ResultsAfter 2 months of HFHS consumption, mini-pigs displayed an obese phenotype characterized by high circulating insulin, triglycerides and cholesterol levels. At the tissue level, a general (muscle, adipose tissue, intestine) reduction in the capacity to phosphorylate glucose was observed. This was also supported by the enhanced hepatic gluconeogenesis potential, despite the concomitant normoglycaemia, suggesting that the high circulating insulin levels would be enough to maintain glucose homoeostasis. The HFHS feeding also resulted in a reduced capacity of two other pathways: the de novo lipogenesis, and the ranched-chain amino acids transamination. Finally, the follow-up of the urine metabolome over the time allowed determining breaking points in the metabolic trajectory of the animals.ConclusionsSeveral features confirmed the pertinence of the animal model, including increased body weight, adi-posity and porcine obesity index. At the metabolic level,we observed a perturbed glucose and amino acid metabolism, known to be related to the onset of the obesity. The urine metabolome analyses revealed several metabolic pathways potentially involved in the obesity onset, including TCA (citrate, pantothenic acid), amino acids catabolism (cysteine, threonine, leucine).
Databáze: OpenAIRE
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