Type VI Secretion System in Pathogenic Escherichia coli: Structure, Role in Virulence, and Acquisition
| Autor: | Fernando Navarro-Garcia, Fernando Ruiz-Perez, Mariano Larzabal, Angel Adrian Cataldi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Transferencia de Genes Gene Transfer lcsh:QR1-502 effector proteins Virulence Review medicine.disease_cause genome plasticity Microbiology lcsh:Microbiology Bacteriophage 03 medical and health sciences medicine Pathogenicity Secretion type 6 secretion system Escherichia coli 030304 developmental biology Type VI secretion system Escherichia Coli 0303 health sciences protein translocation Virulencia diarrheagenic E. coli biology Patogenicidad 030306 microbiology Effector Periplasmic space biology.organism_classification Pathogenicity island Diarrhoea Cell biology inner and outer membranes bacterial competition Diarrea |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 10 (2019) Frontiers in microbiology 10 : 1965. (30 August 2019) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 1664-302X |
| Popis: | Bacterial pathogens utilize a myriad of mechanisms to invade mammalian hosts, damage tissue sites, and evade the immune system. One essential strategy of Gram-negative bacteria is the secretion of virulence factors through both inner and outer membranes to reach a potential target. Most secretion systems are harbored in mobile elements including transposons, plasmids, pathogenicity islands, and phages, and Escherichia coli is one of the more versatile bacteria adopting this genetic information by horizontal gene transfer. Additionally, E. coli is a bacterial species with members of the commensal intestinal microbiota and pathogens associated with numerous types of infections such as intestinal, urinary, and systemic in humans and other animals. T6SS cluster plasticity suggests evolutionarily divergent systems were acquired horizontally. T6SS is a secretion nanomachine that is extended through the bacterial double membrane; from this apparatus, substrates are conveyed straight from the cytoplasm of the bacterium into a target cell or to the extracellular space. This nanomachine consists of three main complexes: proteins in the inner membrane that are T4SS component-like, the baseplate complex, and the tail complex, which are formed by components evolutionarily related to contractile bacteriophage tails. Advances in the T6SS understanding include the functional and structural characterization of at least 13 subunits (so-called core components), which are thought to comprise the minimal apparatus. So far, the main role of T6SS is on bacterial competition by using it to kill neighboring non-immune bacteria for which antibacterial proteins are secreted directly into the periplasm of the bacterial target after cell–cell contact. Interestingly, a few T6SSs have been associated directly to pathogenesis, e.g., roles in biofilm formation and macrophage survival. Here, we focus on the advances on T6SS from the perspective of E. coli pathotypes with emphasis in the secretion apparatus architecture, the mechanisms of pathogenicity of effector proteins, and the events of lateral gene transfer that led to its spread. Instituto de Biotecnología Fil: Navarro-García, Fernando. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Biología Celular; México Fil: Ruiz-Perez, Fernando. University of Virginia School of Medicine. Department of Pediatrics; Estados Unidos Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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