Culex Species (Diptera: Culicidae) from Central Argentina: Identification by Electrophoretic Zymograms and Genetic Relationships

Autor: M. S. Sabattini, S. G. Humeres, R. Sereno, Cristina N. Gardenal, Walter Ricardo Almirón
Rok vydání: 1990
Předmět:
Zdroj: Journal of Medical Entomology. 27:784-788
ISSN: 1938-2928
0022-2585
DOI: 10.1093/jmedent/27.5.784
Popis: Starch gel electrophoretic zymograms were used for the taxonomic separation of mosquitoes of the genus Culex , possible vectors of encephalitis in Argentina. Catalase, hexokinase, malic enzyme, isocitrate and malate dehydrogenases, and leucine aminopeptidase were species markers that allowed the clear identification of individual Culex apicinus Philippi, Cx. bidens Dyar, Cx. brethesi Dyar, Cx. declarator Dyar and Knab, Cx. dolosus Arribalzaga, Cx. maxi Dyar, Cx. quinquefasdatus Say, Cx. saltanensis Dyar, and Cx. tatoi Casal & Garcia. Intraspecific polymorphism was revealed but did not preclude species separation. On the basis of allele frequencies, an index of genetic distance between species was calculated. Genetic affinities determined by electrophoretic data agreed with taxonomic affinities ascertained by conventional systematics. Thus, electrophoretic zymograms proved to be a powerful tool for the study of the genus Culex in Argentina.
Databáze: OpenAIRE