Rapid determination of acetaminophen in physiological fluids by liquid chromatography using SDS mobile phase and ED detection
| Autor: | Samuel Carda-Broch, Maria-Elisa Capella-Peiró, Abhilasha Durgbanshi, Adrià Martinavarro-Domínguez, D. Bose, Mayte Gil-Agustí, Josep Esteve-Romero |
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| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Chromatography Elution Reproducibility of Results Sodium Dodecyl Sulfate General Medicine Urine Reversed-phase chromatography Hydrogen-Ion Concentration High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry chemistry.chemical_compound Blood serum chemistry Calibration Electrochemistry Sodium dodecyl sulfate Quantitative analysis (chemistry) Acetaminophen Chromatography Liquid |
| Zdroj: | Journal of chromatographic science. 43(6) |
| ISSN: | 0021-9665 |
| Popis: | Acetaminophen is determined in serum and urine samples by a rapid, sensitive, and precise chromatographic method without any pretreatment step in a C18 column using a pure micellar mobile phase of 0.02M sodium dodecyl sulfate at pH 7. Acetaminophen is eluted in less than 5 min with no interference of the protein band. The use of electrochemical and UV detection is compared. Linearities (r > 0.999), as well as intra- and interday precision, are studied in the validation of the method. Limits of detection (LOD) are also calculated to be 0.56, 0.83, and 0.74 ng/mL in micellar solution, serum, and urine using electrochemical detection. The developed micellar liquid chromatographic method is useful for the quantitation of acetaminophen in serum and urine. Recoveries in the biological matrices are in the 98-107% range and results are compared with those obtained using a reference method. Drug excretion (in urine) and serum distribution are studied in several healthy volunteers, and no interference from metabolites is found. The developed procedure can be applied in routine analyses, toxicology, and therapeutic monitoring. |
| Databáze: | OpenAIRE |
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