Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili
| Autor: | Lasta Kocjancic Curty, Thilo Köhler, Francisco Barja, Jean-Claude Pechère, Christian van Delden |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Fimbria
Mutant Structure and Function Swarming (honey bee) Swarming motility medicine.disease_cause Disaccharides Rhamnose Pilus Rhamnose/analogs & derivatives/metabolism Asparagine Disaccharides/metabolism ddc:616 musculoskeletal neural and ocular physiology Flagella/ physiology/ultrastructure food and beverages DNA-Binding Proteins Flagella Pseudomonas aeruginosa Fimbriae Proteins Fimbriae Bacterial/ physiology Signal Transduction Proline Nitrogen Movement Transcription Factors/genetics Glutamic Acid Biology Flagellum Microbiology Trans-Activators/genetics Surface-Active Agents Bacterial Proteins medicine Decanoates/metabolism Flagellin/genetics Histidine Molecular Biology Surface-Active Agents/metabolism Aspartic Acid Bacterial Proteins/genetics Decanoates biochemical phenomena metabolism and nutrition equipment and supplies Culture Media Microscopy Electron Fimbriae Bacterial Mutation Trans-Activators bacteria Pseudomonas aeruginosa/growth & development/ physiology DNA-Binding Proteins/genetics Flagellin Transcription Factors |
| Zdroj: | Journal of Bacteriology, Vol. 182, No 21 (2000) pp. 5990-5996 |
| ISSN: | 0021-9193 |
| Popis: | We describe swarming in Pseudomonas aeruginosa as a third mode of surface translocation in addition to the previously described swimming and twitching motilities. Swarming in P. aeruginosa is induced on semisolid surfaces (0.5 to 0.7% agar) under conditions of nitrogen limitation and in response to certain amino acids. Glutamate, aspartate, histidine, or proline, when provided as the sole source of nitrogen, induced swarming, while arginine, asparagine, and glutamine, among other amino acids, did not sustain swarming. Cells from the edge of the swarm were about twice as long as cells from the swarm center. In both instances, bacteria possessing two polar flagella were observed by light and electron microscopy. While a fliC mutant of P. aeruginosa displayed slightly diminished swarming, a pilR and a pilA mutant, both deficient in type IV pili, were unable to swarm. Furthermore, cells with mutations in the las cell-to-cell signaling system showed diminished swarming behavior, while rhl mutants were completely unable to swarm. Evidence is presented for rhamnolipids being the actual surfactant involved in swarming motility, which explains the involvement of the cell-to-cell signaling circuitry of P. aeruginosa in this type of surface motility. |
| Databáze: | OpenAIRE |
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