The effect of iodination on antifibrinogen antibodies with respect to precipitating and adsorption activities
| Autor: | Robert B. Fritz, Sylvester Lassiter, Eugene D. Day |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Globulin
Swine chemistry.chemical_element Physical Therapy Sports Therapy and Rehabilitation Iodine Fibrinogen Fibrin Antibodies Antigen-Antibody Reactions Antigen Iodine Isotopes medicine Animals Radiometry Antiserum biology Rehabilitation General Medicine Precipitin Precipitins chemistry Biochemistry biology.protein Adsorption Rabbits gamma-Globulins Antibody medicine.drug |
| Zdroj: | Immunochemistry. 4(5) |
| ISSN: | 0019-2791 |
| Popis: | Antiserum globulins were prepared from four individual rabbit antisera against porcine fibrinogen and iodinated at varying levels. The iodinated globulins were tested for ability (a) to be precipitated by porcine fibrinogen and (b) to be adsorbed by porcine fibrin. At each iodination level loss of precipitating activity in a given antiserum globulin was comparable to loss of adsorbing activity. From these data it was concluded that the effect of iodination upon the precipitating activity of antibodies is primarily upon binding rather than upon aggregation. Loss of activity, whether precipitating or adsorbing, when plotted against iodination level was found to be biphasic in the case of each antiserum globulin—a precipitous linear decline followed by a considerably more gentle linear decline. At the mid-points of equivalence of the precipitin curves the molar antibody/antigen ratios remained essentially constant for each antiserum as iodination levels increased although they varied from 10 to 15 from one antiserum to the next. At infinite antibody excess the molar antibody/antigen ratios (equated to effective antigenic valence) ranged from 40 to 60 for lightly iodinated antiserum globulins but decreased to effective antigenic valences of only 20 to 30 at higher iodination levels. From these data it was concluded that the binding of antibodies to certain antigenic determinants of fibrinogen is very sensitive to iodination but to other determinants is relatively insensitive. |
| Databáze: | OpenAIRE |
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