Biotechnological potential of Zymotis-2 bioreactor for the cultivation of filamentous fungi

Autor: Ynoussa Maiga, Gregoria Mitropoulou, Rayhane Hamrouni, Yiannis Kourkoutas, Josiane Molinet, Nathalie Dupuy, Alina Karachurina, Sevastianos Roussos
Přispěvatelé: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU)
Rok vydání: 2021
Předmět:
Zdroj: Biotechnology Journal
Biotechnology Journal, 2022, 17 (1), pp.2100288. ⟨10.1002/biot.202100288⟩
Biotechnology Journal, Wiley-VCH Verlag, 2021, pp.2100288. ⟨10.1002/biot.202100288⟩
Biotechnology Journal, 2022, 17, pp.2100288. ⟨10.1002/biot.202100288⟩
ISSN: 1860-7314
1860-6768
DOI: 10.1002/biot.202100288⟩
Popis: Background/aim A new prototype of Solid-State Fermentation Bioreactor, namely "Zymotis-2 ",was developed to produce fungal spores. Main methods and major results A fermentation process for fungal spores, and hydrolase enzymes (endo and exoglucanases, amylases) production by Trichoderma asperellum DWG3, Aspergillus. niger G131 and Beauveria bassiana was scaled-up from flasks and glass Raimbault column packed with 20 g of solid substrates (dry weight) to 5 kg of solid substrate by using the new Zymotis-2 bioreactor. Fungi strains growth using a mix of vine shoots, wheat bran, and olive pomace was tested under similar experimental conditions in Zymotis-2 bioreactor, column bioreactor and flasks in a parallel fermentation system. Overall, significant spore production on Zymotis-2 bioreactor was obtained, achieving 22.01 ± 0.01×109 spores/g DM 16.30 ± 0.07 × 109 spores/g DM, and 3.30 ± 0.07 × 109 spores/g DM for B. bassiana, T. asperellum DWG3, and A. niger G131, respectively. Forced aeration increased the endoglucanases, exoglucanases and amylases activities for T. asperellum DWG3 but B. bassiana and A. niger G131 were affected negatively by the aerated process, showing the lowest enzyme activities. Conclusions and implications In conclusion, a high yield of spores was obtained at 137 h of cultivation time, confirming the validity of the new Zymotis-2 bioreactor to produce virulent spores at low cost by T. asperellum, B. bassiana and A. niger G131. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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