Frizzled-7 signalling controls tissue separation during Xenopus gastrulation
| Autor: | Rudolf Winklbauer, Herbert Steinbeisser, Araceli Medina, Rajeeb K. Swain |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Frizzled
Mesoderm animal structures Embryo Nonmammalian Xenopus Gene Expression Ectoderm Receptors Cell Surface Xenopus Proteins Receptors G-Protein-Coupled Xenopus laevis GTP-Binding Proteins medicine Morphogenesis Animals Protein Kinase C beta Catenin chemistry.chemical_classification Multidisciplinary biology Convergent extension Wnt signaling pathway Cell Polarity Gastrula Oligonucleotides Antisense biology.organism_classification Dishevelled Cell biology Gastrulation Fibroblast Growth Factors Cytoskeletal Proteins medicine.anatomical_structure chemistry embryonic structures Trans-Activators Signal Transduction |
| Zdroj: | Nature. 413(6858) |
| ISSN: | 0028-0836 |
| Popis: | Cell signalling through Frizzled receptors has evolved to considerable complexity within the metazoans. The Frizzled-dependent signalling cascade comprises several branches, whose differential activation depends on specific Wnt ligands, Frizzled receptor isoforms and the cellular context. In Xenopus laevis embryos, the canonical beta-catenin pathway contributes to the establishment of the dorsal-ventral axis. A different branch, referred to as the planar cell polarity pathway, is essential for cell polarization during elongation of the axial mesoderm by convergent extension. Here we demonstrate that a third branch of the cascade is independent of Dishevelled function and involves signalling through trimeric G proteins and protein kinase C (PKC). During gastrulation, Frizzled-7 (Fz7)-dependent PKC signalling controls cell-sorting behaviour in the mesoderm. Loss of zygotic Fz7 function results in the inability of involuted anterior mesoderm to separate from the ectoderm, which leads to severe gastrulation defects. This result provides a developmentally relevant in vivo function for the Fz/PKC pathway in vertebrates. |
| Databáze: | OpenAIRE |
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