Total Saponins from Discorea nipponica Ameliorate Urate Excretion in Hyperuricemic Mice
| Autor: | Qi Zhou, Fang Lu, Dong-hua Yu, Chong Zhang, Shu-min Liu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Uricosuric Organic anion transporter 1 Glucose Transport Proteins Facilitative Organic Anion Transporters Pharmaceutical Science Allopurinol Mice Inbred Strains Hyperuricemia Organic Anion Transporters Sodium-Independent Pharmacology Kidney Analytical Chemistry Excretion chemistry.chemical_compound Organic Anion Transport Protein 1 Synovial Fluid Drug Discovery medicine Animals Rats Wistar biology Dioscorea Organic Chemistry Glucose transporter Kidney metabolism Saponins medicine.disease Uric Acid Disease Models Animal Gene Expression Regulation Complementary and alternative medicine chemistry Biochemistry Creatinine biology.protein Molecular Medicine Uric acid Drugs Chinese Herbal medicine.drug |
| Zdroj: | Planta Medica. 80:1259-1268 |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | Rhizoma Dioscoreae Nipponicae, from Discorea nipponica, is a widely used traditional Chinese herb. It is used to treat arthroncus, arthrodynia, and arthritis. Hyperuricemia is an important foundation of gouty arthritis. The current study was aimed at investigating whether the effects of total saponins from Rhizoma Dioscoreae Nipponicae on hyperuricemia were due to renal organic ion transporters in potassium oxonate-induced hyperuricemia mice. Hyperplasia of synovial cells prepared from Wistar rats was induced by IL-1β (1 × 104 µg/mL). MTT was used and to screen active components in the inhibition of hyperplasia by total saponins from Rhizoma Dioscoreae Nipponica, individual pure compounds, and different combinations of these compounds. Sixty Kun Ming mice were randomly divided into six groups: normal, model, allopurinol (40 mg/kg), and three total saponins groups receiving dose (600 mg/kg), middle (300 mg/kg), and low doses (60 mg/kg). Hyperuricemic mice were induced with potassium oxonate (300 mg/kg) intragastrically. The total saponins were given six days and the positive drug allopurinol was given one day before inducing hyperuricemia. The serum and urine levels of uric acid and creatinine and the fractional excretion of uric acid were measured in normal and hyperuricemic mice treated with Rhizoma Dioscoreae Nipponicae and allopurinol. The mRNA and protein levels of the mouse urate transporter 1, glucose transporter 9, organic anion transporter 1, and organic anion transporter 3 were analyzed by real-time-PCR and Western blotting methods, respectively. Total saponins from Rhizoma Dioscoreae Nipponicae could effectively reverse potassium oxonate-induced alterations in renal mouse urate transporter 1, glucose transporter 9, organic anion transporter 1, and organic anion transporter 3 mRNA and protein levels, resulting in enhancement of renal urate excretion in mice. These findings suggested that the total saponins from Rhizoma Dioscoreae Nipponicae had a uricosuric effect on the regulation of renal organic ion transporters in hyperuricemic animals. |
| Databáze: | OpenAIRE |
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