Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy
| Autor: | Shuchen Guo, Xiao-Bin Jia, Juan Chen, Weiquan Bu, Bing Yang, Long Wang, Liang Feng, Chang Li, Xiaobin Tan, Wenbo Ding, Yuan-pei Lian, Mao-Mao Zhu, Qinglin Lv |
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| Rok vydání: | 2021 |
| Předmět: |
Glycation End Products
Advanced Male Antioxidant medicine.medical_treatment 02 engineering and technology Pharmacology Paeonia Biochemistry Diabetes Mellitus Experimental RAGE (receptor) Rats Sprague-Dawley Diabetic nephropathy 03 medical and health sciences Western blot Polysaccharides Structural Biology In vivo Human Umbilical Vein Endothelial Cells medicine Animals Humans Diabetic Nephropathies Molecular Biology 030304 developmental biology 0303 health sciences medicine.diagnostic_test Chemistry General Medicine 021001 nanoscience & nanotechnology medicine.disease In vitro Rats Tubulointerstitial fibrosis Immunohistochemistry 0210 nano-technology Drugs Chinese Herbal |
| Zdroj: | International Journal of Biological Macromolecules. 176:589-600 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.02.062 |
| Popis: | This study aimed to investigate the structure of a new heteropolysaccharide (MC-Pa) from Moutan Cortex (MC), and its protection on diabetic nephropathy (DN). The MC-Pa composed of D-glucose and L-arabinose (3.31:2.25) was characterized with homogeneous molecular weight of 1.64 × 105 Da, and the backbone was 4)-α-D-Glcp-(1 → 5-α-L-Araf-(1 → 3,5-α-L-Araf-(1→, branched partially at O-3 with α-L-Araf-(1 → residue with methylated-GC–MS and NMR. Furthermore, MC-Pa possessed strong antioxidant activity in vitro and inhibited the production of ROS caused by AGEs. In vivo, MC-Pa could alleviate mesangial expansion and tubulointerstitial fibrosis of DN rats in histopathology and MC-Pa could decrease significantly the serum levels of AGEs and RAGE. Western blot and immunohistochemical analysis showed that MC-Pa can reduce the expression of main protein (FN and Col IV) of extracellular-matrix, down-regulate the production of inflammatory factors (ICAM-1 and VCAM-1), and therefore regulate the pathway of TGF-β1. The above indicated that MC-Pa has an improving effect on DN. |
| Databáze: | OpenAIRE |
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