Carriers based on poly-3-hydroxyalkanoates containing nanomagnetite to trigger hormone release
| Autor: | Rubén Jesus Sánchez Rodríguez, Thatiane Teixeira Mendonça, Mayara de Freitas e Castro, Luiziana Ferreira da Silva, José Gregório Cabrera Gomez |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Thermogravimetric analysis
Polyesters Hydroxybutyrates Infrared spectroscopy 02 engineering and technology Biochemistry law.invention 03 medical and health sciences chemistry.chemical_compound X-Ray Diffraction Structural Biology law BACTÉRIAS GRAM-NEGATIVAS Spectroscopy Fourier Transform Infrared Particle Size Molecular Biology Progesterone 030304 developmental biology Drug Carriers 0303 health sciences Calorimetry Differential Scanning biology Comonomer Temperature Water General Medicine Hormone release 021001 nanoscience & nanotechnology biology.organism_classification Ferrosoferric Oxide chemistry Burkholderia sacchari Thermogravimetry Drug delivery Emulsion Nanoparticles Emulsions Electron microscope Crystallization 0210 nano-technology Oils Nuclear chemistry |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.10.203 |
| Popis: | Poly-3-hydroxybutyrate (P(3HB)) and poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (P(3HB-co-3HHx)) are biocompatible and bioabsorbable biopolymers produced by different bacteria with potential for drug delivery in thermo-responsive magnetic microcarriers. Microparticles of P(3HB) and P(3HB-co-3HHx), with 5.85% mol of 3HHx, produced by Burkholderia sacchari, containing nanomagnetite (nM) and lipophilic hormone were prepared by simple emulsion (oil/water) technique leading to progesterone (Pg) encapsulation efficiency higher than 70% and magnetite loads of 3.1 and 2.3% (w/w) for P(3HB)/nM/Pg and P(3HB-co-3HHx)/nM/Pg, respectively. These formulations were characterized by Infrared spectroscopy, X-ray diffraction, Thermal gravimetric analysis and Electron microscopy (TEM, SEM) techniques. The P(3HB)/nM/Pg and P(3HB-co-3HHx)/nM/Pg microparticles presented spherical geometry with wrinkled surfaces and average size between 2 and 40 μm for 90% of the microparticles. The release profiles of the P(3HB)/nM/Pg and P(3HB-co-3HHx)/nM/Pg formulations showed a hormone release trigger (6.9 and 11.1%, respectively) effect induced by oscillating external magnetic field (0.2 T), after 72 h. Progesterone release in non-magnetic tests with P(3HB-co-3HHx)/nM/Pg revealed a slight increment (5.6%) in relation to P(3HB)/nM/Pg. The experimental release of the P(3HB)/nM/Pg and P(3HB-co-3HHx)/nM/Pg samples presented a good agreement with Higuchi model. The 3HHx comonomer content improves the hormone release of the P(3HB-co-3HHx)/nM/Pg formulation with potential for application to synchronize the estrous cycle. |
| Databáze: | OpenAIRE |
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